catalog number :
MBS346006
products full name :
CD57 Antibody
products short name :
[CD57]
products gene name :
[CD57]
form :
Liquid; Tris Buffer, ph7.4, 1% BSA, <0.1% NaN3
storage stability :
Store at 2-8 degree C. Do not freeze.
tested application :
Immunohistochemistry (IHC)
app notes :
Dilution Range: 1:100 - 1:200
image1 heading :
Immunohistochemistry (IHC)
other info2 :
IHC Positive Control: Tonsil. IHC Suggested Protocol: 1. Tissues are fixed with 10% neutral buffered formalin, cut to ~4um, and placed on a positively charged glass slide. 2. The prepared slide is baked for at least 30 minutes and at most 24 hours at 55-65 degree C. 3. Pretreatment - Perform heat-induced epitope retrieval (HIER) at pH 6 for 10 to 30 minutes. 4. Block – Block with peroxidase solution for 5 minutes at RT. 5. Primary Ab – Dilute antibodies 1:100 – 1:200 and incubate for 10 - 30 minutes at RT. 6. Secondary Ab - Incubate for 20 - 30 minutes at RT. 7. Substrate - Incubate DAB or Fast Red for 5 - 10 minutes at RT. 8. Counterstain - Counterstain with hematoxylin for 30 seconds to 5 minutes, rinse with distilled water and blueing solution for 30 seconds. 9. Dehydrate and apply coverslip. (Each researcher should optimize the protocol for their experimental model system.)
products description :
CD57 (Cluster of Differentiation 57, also known as NK-1) is a marker for natural killer (NK) cells and some T-lymphocytes, and can be used to indicate tumors of neuroendocrine origin. CD57 is also detectable in ganglioneuroma and prostate carcinoma.