ELISA/assay

Human IL-1 beta ELISA Set

MBS335540

1 x96 Wells

205 USD

ELISA Set

Human IL-1 beta ELISA Set

IL-1 beta

IL-1 b

interleukin-1 beta proprotein; Interleukin-1 beta; interleukin-1 beta; interleukin 1, beta; Catabolin

IL-1 beta

IL1B; IL1B; IL-1; IL1F2; IL1-BETA; IL1F2; IL-1 beta

IL1B_HUMAN

Human. No cross reactivity with other human cytokines.

269

Recognizes both natural (pro and mature) and recombinant human IL-1b

Intended Uses: The IL1beta ELISA Set is intended for use in a 'do it yourself' solid phase sandwich ELISA for the in vitro qualitative and quantitative determination of IL1beta in supernatants, buffered solutions, serum, plasma samples and other body fluids. This assay will recognise both natural and recombinant human IL1beta.

Sensitivity: The sensitivity, minimum detectable dose of this IL1beta antibody pair was determined using the IL1beta ELISA kit (which contains the same antibodies) and was found to be <6.5pg/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed in 6 independent experiments.

Human ELISA Sets

MyBioSource ELISA Sets include capture and biotinylated detection antibody, one standard per plate, Streptavidin-HRP, TMB, and detailed procedure including buffer composition.

Haller D. et al., Clin. Diagn. Lab. Immunol., 2002; 9(3): 649 - 657. Kankkunen, P. et al., J. Immunol.,2009; 182(10): 6418-6425. Kankkunen, P. et al., J. Immunol., 2010; 184(11): 6335-6342

10835145

NP_000567.1

NM_000576.2

30,748 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); C-type Lectin Receptors (CLRs) Pathway (1269303)

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q14. Cellular Component: extracellular space; extracellular region; cytosol; secretory granule. Molecular Function: protein domain specific binding; interleukin-1 receptor binding; cytokine activity. Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; negative regulation of MAP kinase activity; positive regulation of nitric oxide biosynthetic process; activation of MAPK activity; positive regulation of transcription, DNA-dependent; positive regulation of interleukin-2 biosynthetic process; germ cell programmed cell death; negative regulation of insulin receptor signaling pathway; positive regulation of lipid catabolic process; positive regulation of NF-kappaB import into nucleus; fever; positive regulation of membrane protein ectodomain proteolysis; response to carbohydrate stimulus; activation of NF-kappaB transcription factor; cell-cell signaling; positive regulation of phagocytosis; positive regulation of T cell mediated immunity; positive regulation of T cell proliferation; neutrophil chemotaxis; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of mitosis; smooth muscle adaptation; interleukin-1 beta production; positive regulation of interleukin-6 production; positive regulation of angiogenesis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; negative regulation of lipid metabolic process; sequestering of triacylglycerol; positive regulation of histone phosphorylation; apoptosis; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of protein export from nucleus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; negative regulation of lipid catabolic process; hyaluronan biosynthetic process; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; response to ATP; positive regulation of interferon-gamma production; positive regulation of chemokine biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of fever; positive regulation of histone acetylation; immune response; positive regulation of protein amino acid phosphorylation; regulation of insulin secretion; embryo implantation. Disease: Gastric Cancer, Hereditary Diffuse

1 x96 Wells

205 USD

5x96 Wells

405

10x96 Wells

505

15x96 Wells

575

20x96 Wells

675