ELISA/assay

Human IFN gamma HS ELISA Kit

MBS335502

1 x48 Wells (Pre-Coa

385 USD

ELISA Kit

Human IFN gamma HS ELISA Kit

IFN gamma

IFN-g HS

interferon gamma; Interferon gamma; interferon gamma; interferon, gamma; Immune interferon

IFN gamma

IFNG; IFNG; IFG; IFI; IFN-gamma

IFNG_HUMAN

Human. No cross reactivity with other human cytokines.

166

Ten specificities were tested with concentrations higher than IFNgamma curve concentrations. No cross reaction was observed for concentrations ranging from 2500 to 78.12 pg/ml for IL-1alpha, IL-2, IL-8, IL-12p40, TNFalpha, CD95 Fas, TRAIL, ICAM-1, Gp130 and GM-CSF.

Samples: Human sera, plasmas, buffered solutions or cell culture media. The assay will recognize both natural and recombinant human IFNgamma. Assay Type: Sandwich. Sensitivity: The sensitivity, minimum detectable dose of Human IFNgamma using this Human IFNgamma High Sensitivity ELISA kit was found to be <0.69 pg/ml. This was determined by adding 3 standard deviations to the mean OD obtained when the zero standard was assayed 40 times.

Human ELISA Kit

Principle of the Assay: The IFNgamma Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IFNgamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFNgamma concentrations and unknowns are pipetted into these wells. During the first incubation, the IFNgamma antigen and a biotinylated monoclonal antibody specific for IFNgamma are successively incubated. After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotine-Tyramine reagent. Under the action of HRP, a biotine polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotine is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IFNgamma present in the samples.

56786138

NP_000610.2

NM_000619.2

P01579

19,348 Da

ATF-2 Transcription Factor Network Pathway (138006); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485)

This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family. Protein type: Secreted; Cytokine; Secreted, signal peptide; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12q14. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: interferon-gamma receptor binding; cytokine activity. Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-23 production; positive regulation of interleukin-12 production; apoptosis; positive regulation of osteoclast differentiation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; defense response to protozoan; positive regulation of tumor necrosis factor production; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; positive regulation of chemokine biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; cell motility; positive regulation of neuron differentiation; regulation of insulin secretion. Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Tuberous Sclerosis 2; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To

1 x48 Wells (Pre-Coated)

385 USD

1x96 Wells (Pre-Coated)

530

2x96 Wells (Pre-Coated)

830