product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human CD25 ELISA Kit
catalog :
MBS335497
quantity :
1 x48 Wells (Pre-Coa
price :
305 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS335497
products type :
ELISA Kit
products full name :
Human CD25 ELISA Kit
products short name :
CD25
products name syn :
CD25 / IL-2R
other names :
CD25; Peripheral myelin protein 22; Protein CD25; SR13 myelin protein; Schwann cell membrane glycoprotein; SAG
products gene name :
CD25
other gene names :
Pmp22; Cd25; Pmp-22; PMP-22; SAG
uniprot entry name :
PMP22_RAT
reactivity :
Human. No cross reactivity with other human soluble receptors.
sequence length :
160
specificity :
Recognizes both natural and recombinant human soluble IL-2R
other info1 :
Samples: Supernatants, buffered solutions or serum and plasma samples. Sensitivity: The sensitivity or minimum detectable dose of CD25 using this CD25 ELISA kit was found to be 32.5pg/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed in 6 independent experiments.
other info2 :
Intra-assay Precision: Reproducibility within the assay will be evaluated in three independent experiments. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD25. 2 standard curves were run on each plate The overall intra-assay coefficient of variation has been calculated to be 4.5%. Inter-assay Precision: Assay to assay reproducibility within one laboratory will be evaluated in three independent experiments by two technicians. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD25. 2 standard curves were run on each plate. The calculated overall coefficient of variation was 10.3%. Intended Uses: The sCD25 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD25 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD25.
products categories :
Human ELISA Kit
products description :
MyBioSource ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
products references :
Botella-Carretero J. I. et al., Eur. J. Endocrinol., 2005; 153(2): 223 - 230. Dennert, R. et al., Clin. Vaccine Immunol.,2012; 19(8): 1182-1187. Foussat A. et al., Blood, 2001; 98(6): 1678 - 1686
ncbi gi num :
55904
ncbi acc num :
CAA44297.1
uniprot acc num :
P25094
ncbi mol weight :
17,946 Da
size1 :
1 x48 Wells (Pre-Coated)
price1 :
305 USD
size2 :
1x96 Wells (Pre-Coated)
price2 :
470
size3 :
2x96 Wells (Pre-Coated)
price3 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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