catalog number :
MBS335467
products type :
ELISA Kit
products full name :
Human CD138 ELISA Kit
products short name :
CD138
products name syn :
CD138 / SYNDECAN-1
products gene name :
CD138
reactivity :
Human. No cross reactivity with other human soluble molecules
specificity :
Recognizes natural human soluble syndecan-1
other info1 :
Samples: Supernatants, buffered solutions or serum and plasma samples. Sensitivity: The sensitivity or minimum detectable dose of CD138 using this CD138 ELISA kit was found to be 4.94ng/ml. This was determined by adding 2 standard deviations to the mean OD obtained when the zero standard was assayed in 6 independent experiments.
other info2 :
Intra-assay Precision: Reproducibility within the assay will be evaluated in three independent experiments. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD138. 2 standard curves were run on each plate The overall intra-assay coefficient of variation has been calculated to be 6.2%. Inter-assay Precision: Assay to assay reproducibility within one laboratory will be evaluated in three independent experiments by two technicians. Each assay will be carried out with 6 replicates (3 duplicates) in 2 human pooled serum, 2 in RPMI and 2 in standard diluent with samples containing different concentrations of CD138. 2 standard curves were run on each plate. The calculated overall coefficient of variation was 10.2%. Intended Uses: The sCD138 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of sCD138 in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant human sCD138.
products categories :
Human ELISA Kit
products description :
MyBioSource ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
products references :
Annecke, T. et al., Br. J. Anaesth.,2010;104(4): 414-421. Bruegger D. et al., Am J Physiol Heart Circ Physiol, 2005; 289(5):H1993-9. Bruegger, D. et al., J. Thorac. Cardiovasc. Surg.,2009;138(6): 1445-1447.
size1 :
1 x48 Wells (Pre-Coated)
size2 :
1x96 Wells (Pre-Coated)
size3 :
2x96 Wells (Pre-Coated)