ELISA/assay

Rat IFN gamma ELISA Kit

MBS335444

1 x48 Wells (Pre-Coa

290 USD

ELISA Kit

Rat IFN gamma ELISA Kit

IFN gamma

IFN-g

interferon gamma; Interferon gamma; interferon gamma; interferon gamma

IFN gamma

Ifng; Ifng; Ifg; IFN-g; IFN-gamma

IFNG_MOUSE

Rat. No cross reactivity with other rat cytokines

155

Assay Type: Sandwich. Sensitivity: Less than 10 pg/ml

Rat ELISA Kit

Intended Uses: The Rat IFNgamma ELISA is to be used for the in-vitro quantitative determination of rat Interferon-gamma. The assay will recognize both natural and recombinant Rat IFN-gamma. Principle of the Assay The Rat IFN-gamma Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for rat IFN-gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN-gamma concentrations and unknowns are pipetted into these wells. During the first incubation, the rat Interferon-gamma antigen and a biotinylated monoclonal antibody specific for rat IFN-gamma are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of rat IFN-gamma present in the samples.

33468859

NP_032363.1

NM_008337.3

P01580

17,907 Da

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (83316); Allograft Rejection Pathway (535); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795)

This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]

IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family. Protein type: Secreted; Membrane protein, integral; Secreted, signal peptide; Cytokine. Cellular Component: extracellular space; cell; extracellular region; intracellular; external side of plasma membrane. Molecular Function: protein binding; interferon-gamma receptor binding; cytokine activity. Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of cell adhesion; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; positive regulation of membrane protein ectodomain proteolysis; regulation of growth; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; response to virus; positive regulation of tumor necrosis factor production; defense response to protozoan; inflammatory cell apoptosis; positive regulation of tyrosine phosphorylation of Stat1 protein; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-12 production; apoptosis; positive regulation of interleukin-6 biosynthetic process; sensory perception of mechanical stimulus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of transcription, DNA-dependent; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; regulation of immune response; adaptive immune response; unfolded protein response; humoral immune response; antigen processing and presentation; positive regulation of chemokine biosynthetic process; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; protein import into nucleus, translocation; immune response; positive regulation of neuron differentiation; regulation of insulin secretion

1 x48 Wells (Pre-Coated)

290 USD

1x96 Wells (Pre-Coated)

410

2x96 Wells (Pre-Coated)

515