antibody

Mouse Anti-Human CD44 Monoclonal Antibody, Azide Free Clone B-F24

MBS335060

0.2 mg

310 USD

monoclonal

mouse

nonconjugated

[B-F24]

ELISA, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

Antibody

Mouse Anti-Human CD44 Monoclonal Antibody, Azide Free Clone B-F24

[CD44]

[CD44, PGP-1, H-CAM]

[CD44, partial; CD44 antigen; CD44 antigen; CD44 molecule (Indian blood group); CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; PGP-I; CD_antigen: CD44]

[CD44]

[CD44; CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I]

CD44_HUMAN

Monoclonal

Mouse IgG1 Kappa ligth chain

[B-F24]

Mouse

Human

43

Recognises the Hyaluronate Cell Adhesion Molecule (H-CAM), a 85-250 kDa protein

Ion exchange chromatography

Phosphate-buffered saline. Sterile-filtered through 0.22 um. Carrier and preservative free

Store at +2-8° C for 12 months. For longer storage, freeze aliquots.

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Functional Assay, ELISA (EIA)

Testing Data

Workshop: VI

Antigen: CD44 / PGP-1. Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells. Immunisation: U937 cell line. Biological Activity: Triggers Tt-lymphocyte aggregation. Enhances Hyaluronan binging to CD44.

Lefevre et.al J Immunol 1999

685122

AAB30429.1

P16070

Cell Surface Interactions At The Vascular Wall Pathway (1269373); Cytokine Signaling In Immune System Pathway (1269310); Degradation Of The Extracellular Matrix Pathway (1270257); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Extracellular Matrix Organization Pathway (1270244); Glycosaminoglycan Metabolism Pathway (1269972); Hematopoietic Cell Lineage Pathway (83078)

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

CD44: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Interacts with PKN2. Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. 19 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 11p13. Cellular Component: Golgi apparatus; cell surface; focal adhesion; integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: collagen binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; hyaluronic acid binding; hyalurononglucosaminidase activity. Biological Process: extracellular matrix organization and biogenesis; Wnt receptor signaling pathway; positive regulation of heterotypic cell-cell adhesion; glycosaminoglycan metabolic process; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; pathogenesis; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; hyaluronan catabolic process; extracellular matrix disassembly; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell-cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; ureteric bud branching; cartilage development; carbohydrate metabolic process; blood coagulation; leukocyte migration; hyaluronan metabolic process; healing during inflammatory response; negative regulation of apoptosis. Disease: Blood Group, Indian System

0.2 mg

310 USD