antibody

Mouse Anti-Human Perforin Monoclonal Antibody, Azide Free Clone B-D48

MBS335051

0.2 mg

310 USD

monoclonal

mouse

nonconjugated

[B-D48]

western blot, ELISA, immunohistochemistry, flow cytometry, FACS, enzyme immunoassay

Antibody

Mouse Anti-Human Perforin Monoclonal Antibody, Azide Free Clone B-D48

[Perforin]

[Perforin]

[perforin; Perforin-1; perforin-1; perforin 1 (pore forming protein); Cytolysin; Lymphocyte pore-forming protein; PFP]

[PRF1; PRF1; P1; PFP; FLH2; PFN1; HPLH2; PFP; P1; PFP]

PERF_HUMAN

Monoclonal

IgG1 Kappa light chain

[B-D48]

Mouse

Human

Recognizes human Perforin, a 70 kDa protein

Ion exchange chromatography

Phosphate-buffered saline. Sterile-filtered through 0.22 um. Carrier and preservative free

Store at +2-8°C for 12 months. For longer storage, freeze aliquots.

Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC) on frozen sections.

Flow Cytometry (FC/FACS)

Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells. Immunization: Natural human Perforin

de Rosa, S.C. et al.,J. Immunol.,2011;187: 3391-3401

189847

AAA60065.1

P14222

Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Apoptosis Pathway (198797); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Caspase Cascade In Apoptosis Pathway (137974); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Graft-versus-host Disease Pathway (83124); Graft-versus-host Disease Pathway (536)

The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

PRF1: Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. Monomer, as sobluble protein. Homooligomer. Oligomerization is required for pore formation. Repressed by contact with target cells. Belongs to the complement C6/C7/C8/C9 family. Protein type: Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 10q22. Cellular Component: membrane; cytoplasmic membrane-bound vesicle; integral to membrane; extracellular region; plasma membrane. Molecular Function: protein binding; calcium ion binding; wide pore channel activity. Biological Process: formation of immunological synapse; apoptosis; cytolysis; defense response to tumor cell; cellular defense response; immune response to tumor cell; transmembrane transport; defense response to virus; protein homooligomerization. Disease: Lymphoma, Non-hodgkin, Familial; Aplastic Anemia; Hemophagocytic Lymphohistiocytosis, Familial, 2

0.2 mg

310 USD