catalog number :
MBS330007
products full name :
Anti-phospho-S6 peptide, Peroxidase Conjugates (HRP)
products short name :
S6 peptide
products name syn :
Rabbit anti-phospho-S6 peptide antibody(Peroxidase Conjugates (HRP))
other names :
protein phosphatase; Serine/threonine-protein phosphatase PP1(4.8)
products gene name :
S6 peptide
uniprot entry name :
PP11_TRYBB
specificity :
Recognize only the phophophorylated S6 peptide product of S6K, not cross-react with the non-phosphorylated S6 peptide substrate
purity :
Affinity purified with phospho-S6 peptide KRRRLApSLR on Agarose and affinity adsorbed with non-phosphorylated S6 peptide
form :
250 ug/mL in PBS, 50% glycerol:
storage stability :
Store at -20 degree C, stable for up to 12 months
tested application :
ELISA (EIA), Western Blot (WB)
app notes :
ELISA, WB, non-radioactive S6K kinase assay
other info1 :
Immunogen: phosphorylated S6 peptide KRRRLApSLR-KLH conjugates
other info2 :
Method of Conjugation: Reductive amination with 2/1 molar ratio of HRP/antibody.
products description :
S6 peptide (KRRRLASLR) is a substrate of S6K and ILK. Affinity purified rabbit polyclonal anti-pS6 antibodies were developed with phosphorylated S6 (KRRRLApSLR) and absorbed with non-phosphorylated S6 peptide (KRRRLASLR). This antibody is conjugated to horse-radish peroxidase through reductive amination. This antibody has been utilized for non-raioactive p70 S6K kinase assay.
ncbi acc num :
CAA36959.1
ncbi mol weight :
39,373 Da
uniprot summary :
Catalytic activity: A phosphoprotein + H2O = a protein + phosphate. Cofactor: Binds 1 iron ion per subunit . By similarity.Binds 1 manganese ion per subunit . By similarity. Miscellaneous: Trypanosoma brucei contains two PP1 genes which are highly similar. Sequence similarities: Belongs to the PPP phosphatase family. PP-1 subfamily.