protein

CK-BB, Recombinant

MBS319257

1 mg

490 USD

Recombinant Protein

CK-BB, Recombinant

Creatine Kinase BB (CK-BB) Recombinant

Creatine Kinase BB (CK-BB) Isoenzyme Recombinant

Creatine kinase B-type; Creatine kinase B-type; creatine kinase B-type; creatine kinase-B; creatine kinase B chain; creatine kinase, brain; B-CK; Creatine kinase B chain

CKB; CKB; B-CK; CKBB; CKBB

KCRB_HUMAN

Pichia pastoris

381

Creatine Kinase BB (CK-BB) Recombinant

>95% pure (SDS-PAGE). Purified under non-denaturing conditions.

Purified, Liquid

3.0mg/ml (Coomassie Plus)

Store at -70 degree C or below. Aliquot to avoid multiple freeze/thaw cycles.

ELISA

Suitable for use in ELISA. Each laboratory should detemine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Inactivation: Not applicable. Activity: 1,922 IU/mg (Sigma CPK Procedure No 45). One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37 degree C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction.

Buffer: 10mM Bis-Tris-HCl, 0.5mM DTT, 0.5mM EDTA, 50% glycerol, pH 6.0. Preservative: No. Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product. Important Note: Centrifuge before opening to ensure complete recovery of vial contents.

Purified Cardiac Markers

Full length Creatine Kinase BB isoenzyme with amino acid sequence identical to the native enzyme. CK-BB is a 47 kDa dimeric protein comprised of 2 identical B subunits. Purified in the enzymatically active form. Reacts with polyclonal antibodies to BB isoenzyme in ELISA.

125294

P12277.1

P12277

42,644 Da

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Metabolic Pathways (132956); Metabolism Of Amino Acids And Derivatives Pathway (106169); Urea Cycle And Metabolism Of Amino Groups Pathway (198758); Creatine-phosphate Biosynthesis Pathway (138632)

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Catalytic activity: ATP + creatine = ADP + phosphocreatine. Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Subcellular location: Cytoplasm. Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.

1 mg

490 USD