protein

SDF-1a, Recombinant

MBS319019

0.01 mg

265 USD

Recombinant Protein

SDF-1a, Recombinant

Stromal Cell-Derived Factor 1 alpha (SDF-1a) Recombinant

stromal cell-derived factor 1 isoform alpha; CXCL12 protein; stromal cell-derived factor 1; OTTHUMP00000019491; OTTHUMP00000019492; OTTHUMP00000216979; OTTHUMP00000216980; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; chemokine (C-X-C motif) ligand 12

CXCL12; CXCL12; IRH; PBSF; SDF1; TLSF; SDF1A; SDF1B; TPAR1; SCYB12

Q6ICW0_HUMAN

E Coli

Human

Stromal Cell-Derived Factor 1 alpha (SDF-1a) Recombinant

>98% pure (RP-HPLC and SDS-PAGE). Purified by chromatographic techniques. Product is sterile filtered.

Purified, Lyophilized. Reconstitute using sterile deionized water to a concentration >100ug/ml. Further dilutions can be made in other aqueous buffers.

1mg/ml (OD280nm, E0.1% = 1.06) (prior to lyophilization)

The lyophilized product, though stable for 3 weeks at room temperature, is best stored at -20 degree C. After reconstitution, short term (up to 1 week) store at 2 to 8 degree C. Long term, add 0.1% HSA or BSA, aliquot and store at -20 degree C.

Inactivation: Not applicable

Buffer: Lyophilized from water containing no additives. Preservative: No. Lyophilized: Yes

Purified Cytokines and Growth Factors

Recombinant Human Stromal Cell-Derived Factor 1 alpha (SDF-1a). Recombinant Human SDF-1 is a non-glycosylated polypeptide chain containing 68 amino acids and having a molecular weight of 8,004 Da. The sequence of the first five N-terminal amino acids wa

40316924

NP_954637.1

NM_199168.3

Q6ICW0

10,103 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); CXCR4-mediated Signaling Events Pathway (137910); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); G Alpha (i) Signalling Events Pathway (119550)

This gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. This gene product and its receptor CXCR4 can activate lymphocytes and have been implicated in the metastasis of some cancers such as breast cancer. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cell development/differentiation; Secreted; Chemokine. Chromosomal Location of Human Ortholog: 10q11.1. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: growth factor activity; chemokine receptor binding; chemokine activity; CXCR chemokine receptor binding; receptor binding. Biological Process: response to peptide hormone stimulus; positive regulation of dopamine secretion; positive regulation of cell adhesion; blood circulation; adult locomotory behavior; neuron migration; motor axon guidance; chemotaxis; signal transduction; germ cell development; induction of positive chemotaxis; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; regulation of actin polymerization and/or depolymerization; response to virus; patterning of blood vessels; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; response to heat; response to mechanical stimulus; ameboidal cell migration; positive regulation of axon extension involved in axon guidance; response to hypoxia; immune response; positive regulation of endothelial cell proliferation; telencephalon cell migration. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

0.01 mg

265 USD