catalog number :
MBS318202
products type :
Native Protein
products full name :
Human Trypsin
products short name :
Trypsin
other names :
trypsin; Trypsin, alkaline C
uniprot entry name :
TRYC_MANSE
purity :
>95% pure (SDS-PAGE). Shows no chymotrypsin activity when assayed against N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine-p-nitroanilide.
form :
Purified, Lyophilized. Reconstitute in 56.5ul 2mM HCl. Use product immediately after bringing to neutral pH to avoid auto-degradation.
concentration :
Total protein: 1.77mg/ml (prior to lyophilization). Activity: >2.5units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of N-Benzyl-L-arginine-pNA per minute at 25 degree C in 200mM Tris-HCl, pH 7.8, 20mM CaCl2.
storage stability :
Store lyophilized product at -20 degree C. After reconstitution, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.
other info2 :
Buffer: Not applicable. Preservative: No. Lyophilized: Yes. Warnings: All human source materials have tested negative for HIV1, HIV2 and non-reactive for HCV, HBc antibodies and HBsAg by FDA required tests. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tiss
products categories :
Purified Enzymes
ncbi acc num :
AAA29341.1
ncbi mol weight :
27,474 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Catalytic activity: Preferential cleavage: Arg- -Xaa, Lys- -Xaa. Subcellular location: Secreted extracellular space. Tissue specificity: Midgut. Sequence similarities: Belongs to the peptidase S1 family.Contains 1 peptidase S1 domain. Sequence caution: The sequence AAA29341.1 differs from that shown. Reason: Erroneous initiation.