catalog number :
MBS315994
products full name :
Sheep anti Human Elastase
products short name :
Elastase (Neutrophil)
products name syn :
Sheep Antibody to Human Elastase
other names :
elastase; Elastase
uniprot entry name :
Q25510_MANSE
specificity :
Elastase (Neutrophil). Shown to be specific by Western blotting and gel diffusion techniques. Elastase is a serine protease which exhibits endopeptidase activity on many proteins, its principal substrate, however, is elastin and it is unique among proteases in its to hydrolyze native elastin. It is present in the pancreas, leukocytes and serum. Elastase consists of single polypeptide chain with a molecular weight of 29.5kDa.
purity :
Delipidated and if necessary adsorbed to monospecificity by use of solid-phase adsorbants. Following adsorption an immunoglobulin fraction is produced by ion-exchange chromatography and preservatives added.
concentration :
23.68mg/ml. (OD280nm, E^1% = 14.5)
storage stability :
Upon receipt, store at 2 to 8 degree C.
other info1 :
Immunogen: Human Elastase: Purified from neutrophils. Application: Suitable for use in Western blotting. It has been evaluated using reducing conditions and a 10-20% SDS gel. Suitability for use in immnunohistochemica procedures and enzymelinked immunoasorbent assays has been assessed but use in such assays should not necessarily be excluded. The following dilutions hare for guidance only:. Antigen: 6 ug Elatase. Antibody: 1:100. Secondary Antibody: 1:1000 DK-a.Sheep/ Goat immunoglobulins-peroxidase.
other info2 :
Buffer: Glycine buffered saline, pH 7.4. Preservative: 0.099% Sodium azide, 0.1% E-Amino-n-Caproic Acid, 0.01% Benzamidine, 1mM EDTA. Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
products categories :
Polyclonal Antibodies to Enzymes
ncbi acc num :
AAA67842.1
ncbi mol weight :
31,271 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Sequence similarities: Belongs to the peptidase S1 family. RuleBase RU000360