Sheep anti Rat Renin | MyBioSource MBS315812 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Sheep anti Rat Renin

catalog :

MBS315812

quantity :

1 mg

price :

575 USD

clonality :

polyclonal

host :

sheep

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

citations: 2

Reference
Barone S, Xu J, Zahedi K, Brooks M, Soleimani M. Probenecid Pre-treatment Downregulates the Kidney Cl-/HCO3- Exchanger (Pendrin) and Potentiates Hydrochlorothiazide-Induced Diuresis. Front Physiol. 2018;9:849 pubmed publisher
Zahedi K, Barone S, Xu J, Soleimani M. Potentiation of the effect of thiazide derivatives by carbonic anhydrase inhibitors: molecular mechanisms and potential clinical implications. PLoS ONE. 2013;8:e79327 pubmed publisher

product information

catalog number :

MBS315812

products type :

Antibody

products full name :

Sheep anti Rat Renin

products short name :

Renin

products name syn :

Sheep Antibody to Rat Renin

other names :

renin; Renin; renin; OTTHUMP00000034311; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme; renin; Angiotensinogenase

other gene names :

REN; REN; HNFJ2; FLJ10761

uniprot entry name :

RENI_HUMAN

clonality :

Polyclonal

host :

Sheep

reactivity :

Rat

sequence length :

406

specificity :

Renin. Reacts with rat and mouse renin

purity :

Protein G chromatography

form :

Purified, Liquid

concentration :

8.7 mg/ml (OD280nm, E^0.1% = 1.36)

storage stability :

Upon receipt, store at -70 degree C. Avoid multiple freeze/thaw cycles. Shipping: ships on cold packs.

tested application :

EIA/ELISA, Western Blot, Immunohistochemistry

app notes :

Suitable for use in ELISA (1:50,000), Western Blot (1:5,000) and immunohistochemistry (1:1,000). Each laboratory should determined an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

other info1 :

Immunogen: Recombinant Rat Prorenin

other info2 :

Buffer: 0.05M Sodium phosphate, 0.1M Sodium chloride, 1mM EDTA, pH 6.6. Preservative: No. Important Note: Centrifuge before opening to ensure complete recovery of vial contents.

products categories :

Polyclonal Antibodies to Enzymes

products references :

Carilli, C.T., et al., (1988), "Semi-preparative purification of recombinant human renin and prorenin", J. Chromatogr., 444: 203-208. Sielecki, A.R., et al., (1989), "Structure of recombinant human renin, a target for cardiovasc

ncbi gi num :

190994

ncbi acc num :

AAA60363.1

uniprot acc num :

P00797

ncbi mol weight :

45,057 Da

ncbi pathways :

ACE Inhibitor Pathway (198763); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486)

ncbi summary :

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq]

uniprot summary :

Function: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Catalytic activity: Cleavage of Leu- -Xaa bond in angiotensinogen to generate angiotensin I. Enzyme regulation: Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Subunit structure: Interacts with ATP6AP2. Ref.14. Subcellular location: Secreted. Membrane. Note: Associated to membranes via binding to ATP6AP2. Involvement in disease: Defects in REN are a cause of renal tubular dysgenesis (RTD) [. MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Ref.17Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [. MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. Ref.18. Sequence similarities: Belongs to the peptidase A1 family. Biophysicochemical propertiesKinetic parameters:KM=1 uM for angiotensinogen (in absence of ATP6AP2)KM=0.15 uM for angiotensinogen (in presence of membrane-bound ATP6AP2)

size1 :

1 mg

price1 :

575 USD

more info or order :

MyBioSource product webpage