Rabbit A' Beta d Galactosidase | MyBioSource MBS315025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit A' Beta d Galactosidase

catalog :

MBS315025

quantity :

10 mg

price :

470 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

Escherichia coli str. K-12 substr. MG1655

application :

enzyme immunoassay, immunohistochemistry - paraffin section

product information

catalog number :

MBS315025

products type :

Antibody

products full name :

Rabbit A' Beta d Galactosidase

products short name :

Beta d Galactosidase (E. coli)

products name syn :

Rabbit Antibody to Beta-d-Galactosidase (E. coli)

other names :

beta-D-galactosidase; Beta-galactosidase; beta-D-galactosidase; Lactase

other gene names :

lacZ; lacZ; ECK0341; JW0335

uniprot entry name :

BGAL_ECOLI

clonality :

Polyclonal

host :

Rabbit

specificity :

Beta d Galactosidase (E. coli). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase (E. coli). Recognizes Beta-galactosidase from E. coli. Cross react

purity :

Delipidation, salt fractionation and ion-exchange chromatography followed by extensive dialysis against the buffer.

form :

Purified, Lyophilized. Reconstitute with 1ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature.

concentration :

10mg/ml (OD280nm) (prior to lyophilization)

storage stability :

Store lyophilized product at 2 to 8 degree C. After reconstitution, store for several weeks at 2 to 8 degree C as an undiluted liquid. Prepare working dilution only prior to immediate use. Long-term, aliquot and store at -20 degree C or below.

tested application :

EIA/ELISA, IEP, Immunohistochemistry - Formalin/Paraffin

other info1 :

Immunogen: Beta-Galactosidase (E. coli)

other info2 :

Buffer: Lyophilized from 0.02M Potassium phosphate, 0.15M Sodium chloride, pH 7.2. Preservative: 0.01% (w/v) Sodium azide. Lyophilized: Yes. Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

products categories :

Polyclonal Antibodies to Enzymes

products references :

Davidson, B.L., et al., (2000), "Recombinant adeno-associated virus type 2, 4 and 5 vectors: Transduction of variant cell types and regions in the mammalian central nervous system", PNAS, 97(7): 3428-3432

ncbi gi num :

16128329

ncbi acc num :

NP_414878.1

ncbi gb acc num :

NC_000913.2

uniprot acc num :

P00722

ncbi mol weight :

116,483 Da

ncbi pathways :

Galactose Metabolism Pathway (1012); Galactose Metabolism Pathway (292); Metabolic Pathways (131985); Other Glycan Degradation Pathway (1052); Other Glycan Degradation Pathway (346); Sphingolipid Metabolism Pathway (1065); Sphingolipid Metabolism Pathway (369); Lactose Degradation III Pathway (192); Lactose Degradation III Pathway (139429)

ncbi summary :

Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527].

uniprot summary :

Catalytic activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687. Cofactor: Binds 2 magnesium ions per monomer. Can also use manganese. Ref.9 Ref.13 Ref.20Binds 1 sodium ion per monomer. Ref.9 Ref.13 Ref.20. Enzyme regulation: Inhibited by phenylethyl thio-beta-D-galactoside (PETG), isopropyl thio-beta-D-galactoside (IPTG), L-ribose, D-galactonolactone, lactose and 2-amino-D-galactose. Ref.24. Subunit structure: Homotetramer. Ref.21. Induction: By allolactose. Ref.8 Ref.24. Sequence similarities: Belongs to the glycosyl hydrolase 2 family. Biophysicochemical propertiesKinetic parameters:The values for the enzymatic assays using manganese as cofactor are very close.KM=0.04 mM for p-nitrophenyl beta-D-galactoside Ref.9 Ref.14 Ref.15 Ref.17 Ref.18 Ref.24KM=0.12 mM for o-nitrophenyl beta-D-galactosideKM=0.15 mM for 2,3-dinitrophenyl beta-D-galactopyranosideKM=0.41 mM for 2,5-dinitrophenyl beta-D-galactopyranosideKM=11.6 mM for p-nitrophenol-alpha-L-arabinopyranosideKM=16.9 mM for p-nitrophenol-beta-D-fucopyranosideKM=34 uM for p-nitrophenyl beta-D-galactoside (with magnesium as cofactor and 30 degrees Celsius)KM=140 uM for o-nitrophenyl beta-D-galactoside (with magnesium as cofactor and 30 degrees Celsius)KM=940 uM for allolactose (with magnesium as cofactor and 30 degrees Celsius)KM=1350 uM for lactose (with magnesium as cofactor and 30 degrees Celsius)Vmax=30.9 umol/min/mg enzyme with lactose as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=49.7 umol/min/mg enzyme with allolactose as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=59.7 umol/min/mg enzyme with p-nitrophenyl beta-D-galactoside as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=360 umol/min/mg enzyme with o-nitrophenyl beta-D-galactoside as substrate (with magnesium as cofactor and 30 degrees Celsius)

size1 :

10 mg

price1 :

470 USD