antibody

Mab to Angiotensin I

MBS312935

0.2 mg

755 USD

monoclonal

mouse

nonconjugated

[B937M]

Antibody

Mab to Angiotensin I

[Angiotensin I]

[Mab to Angiotensin I; Monoclonal Antibody to Human Angiotensin I]

[angiotensinogen preproprotein; Angiotensinogen; angiotensinogen; serpin A8; angiotensin I; angiotensin II; OTTHUMP00000035878; pre-angiotensinogen; alpha-1 antiproteinase, antitrypsin; serine (or cysteine) proteinase inhibitor; angiotensinogen (serpin peptidase inhibitor, clade A, member 8); Serpin A8Cleaved into the following 3 chains:Angiotensin-1; Alternative name(s):; Angiotensin I; Ang IAngiotensin-2; Alternative name(s):; Angiotensin II; Ang IIAngiotensin-3; Alternative name(s):; Angiotensin III; Ang III; Des-Asp[1]-angiotensin II]

[AGT; AGT; ANHU; FLJ92595; FLJ97926; SERPINA8; SERPINA8]

ANGT_HUMAN

m

IgG2a

[B937M]

mouse

Human

Human Angiotensin I. Does not crossreact with angiotensin II, angiotensin III or angiotensinogen in ELISA.

Protein A chromatography

Purified, Liquid

1.0mg/ml

Short term store at 2-8 degree C. Long term aliquot and store at -20 degree C. Prepart working dilution onlyh prior to immediate use. Avoid multiple freeze/thaw cycles.

ELISA

Suitable for use in ELISA. Each laboratory shoudl determined an otimum working titer for use in its particular application. Other applications have not been tesed but use in such assays shoudl not necessarily be excluded.

Immunogen: Synthetic peptide corresponding to amino acids 40-49 (Sequence: P-F-H-L-V-I-H-N-E-S(-C)) of the angiotensinogen precursor (Swiss-Prot entry P01019) conjugated to HMW carrier protein. Affinity Constant: Not determined

Buffer: PBS, pH 7.2. Preservative: 0.09% Sodium azide. Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Monoclonal Antibodies to Hormones and Steroids

MAb to Angiotensin 1. Monoclonal Antibody to Human Angiotensin 1

4557287

NP_000020.1

NM_000029.3

P01019

53,154 Da

ACE Inhibitor Pathway (198763); Adipogenesis Pathway (198832); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Metabolism Of Lipids And Lipoproteins Pathway (160976); MicroRNAs In Cardiomyocyte Hypertrophy Pathway (198784)

The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq]

Function: Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2. Ref.11 Ref.12 Ref.16Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Ref.11 Ref.12 Ref.16Angiotensin-3 stimulates aldosterone release. Ref.11 Ref.12 Ref.16Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1. By similarity. Has vasodilator and antidiuretic effects. By similarity. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. By similarity. Ref.11 Ref.12 Ref.16. Subunit structure: During pregnancy, exists as a disulfide-linked 2:2 heterotetramer with the proform of PRG2 and as a complex (probably a 2:2:2 heterohexamer) with pro-PRG2 and C3dg. Ref.8. Subcellular location: Secreted. Tissue specificity: Expressed by the liver and secreted in plasma. Post-translational modification: Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. Involvement in disease: Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [. MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.Defects in AGT are a cause of renal tubular dysgenesis (RTD) [. MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Ref.26. Sequence similarities: Belongs to the serpin family. Caution: It is uncertain whether Met-1 or Met-10 is the initiator.

0.2 mg

755 USD