MAb to Synaptopodin | MyBioSource MBS312195 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MAb to Synaptopodin

catalog :

MBS312195

quantity :

5 mL

price :

595 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

G1D4

reactivity :

human

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS312195

products type :

Antibody

products full name :

MAb to Synaptopodin

products short name :

Synaptopodin

products name syn :

Monoclonal Antibody to Synaptopodin

other names :

synaptopodin

clonality :

Monoclonal

isotype :

IgG1

clone :

G1D4

host :

Host: Mouse. Source: Cell Culture

specificity :

Recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. Reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Cross-reacts with human, bovine, rat, mouse, guinea pig and gerbil. Does not cross-react with rabbit, frog or chicken.

form :

Supernatant, Liquid

storage stability :

Store at 2 to 8 degree C.

tested application :

Immunofluorescence Assay, Immunohistochemistry - Formalin/Paraffin, Western Blot

app notes :

Suitable for use in immunofluorescence, immunohistochemistry (use undiluted with frozen sections or paraffin-embedded tissue after microwave treatment), and Western blot.

other info1 :

Immunogen: Isolated rat kidney glomeruli. Affinity Constant: Not determined

other info2 :

Buffer: Not applicable. Preservative: 0.01% Sodium azide. Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

products categories :

Monoclonal Antibodies for Neuroscience

products references :

1. Mundel, P., et al., (1991), "Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 kD Protein", J. Histochem. and Cytochem., Vol. 39, No. 8, 1047-1056. 2. Mundel, P., et al., (1995), "Structure and function of podocytes: an update", Anat. Embryol., 192, 385-397. 3. Mundel, P., et al., (1997), "Phenotypic conversion and differention of human and rat podocytes in vitro", J. Am. Soc. Nephrol., 8, 89708-89705. 4. Mundel, P., et al., (1997), "Synaptopodin, an actin-associated protein in telencephalic dendrites and in renal podocytes", J. Cell Biol., Vol 139(1), 193-204. 5. Mundel, P., et al., (1997), "Rearrangements of cytoskeleton and cell contacts induce process formation and postmitotic differentiation of conditionally immortalized mouse podocyte cell lines", Exp. Cell Res., 236, 248-258. 6. Kobayashi, N., et al., (1998), "Non-uniform microtubular polarity, established by CHO1/MKLP1 motor protein, is necessary for process formation of podocytes", J. Cell Biol., 143, 1961-1970. 7. Barisoni, L., et al., (1999), "The dysregulated podocyte phenotype: a novel concept in the pathogenesis of collapsing idiopathic focal segmental glomerulosclerosis and HIV-associated nephropathy", J. Am. Soc. Nephrol., 10, 51-61. 8. Kihara, I., et al., "Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy", Histopathology, 67(6), 537-547. 9. Asanuma, K., et al., (2005), "Synaptopodin regulates the actin-binding activity of alpha-actinin in an isoform-specific manner", J. Clin. Invest., 115, 1188-1198

ncbi gi num :

33323347

ncbi acc num :

AAQ07403.1

uniprot summary :

SYNPO: an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity. Protein type: Actin-binding; Cytoskeletal. Chromosomal Location of Human Ortholog: 5q33.1. Cellular Component: postsynaptic membrane; tight junction; cytoplasm; postsynaptic density; stress fiber; dendritic spine; perikaryon; actin cytoskeleton. Molecular Function: protein binding; actin binding. Biological Process: regulation of stress fiber formation; positive regulation of actin filament bundle formation

size1 :

5 mL

price1 :

595 USD

more info or order :

MyBioSource product webpage