MAb to CD4 | MyBioSource MBS311991 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MAb to CD4

catalog :

MBS311991

quantity :

0.2 mg

price :

405 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

13B8.2

reactivity :

human

application :

western blot, ELISA, neutralization, flow cytometry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS311991

products type :

Antibody

products full name :

MAb to CD4

products short name :

CD4

products name syn :

Monoclonal Antibody to Human CD4

other names :

CD4; CD4 protein; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); OTTHUMP00000238897; T-cell surface antigen T4/Leu-3; CD4 molecule

other gene names :

CD4; CD4; CD4mut; CD4 receptor

uniprot entry name :

Q13969_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

13B8.2

host :

Mouse. Hybridization of NS1 myeloma cells with spleen cells from Balb/c mice.

reactivity :

Human

sequence length :

specificity :

CD4. Reacts with CD4 antigen. Has been assigned to the CD4 cluster of differentiation at the 3rd International Workshop in Boston (1986). The CD4 antigen is present on approximately 45% of peripheral blood lymphocytes (T4 lymphocytes), 80% of thymocytes and with a lower density on 100% of monocytes and on some neutrophils.

form :

Purified, Lyophilized. Reconstitute with 1ml distilled water.

concentration :

0.2mg/ml (prior to lyophilization)

storage stability :

Store lyophilized product at 2 to 8 degree C. After reconstitution aliquot and store at 2 to 8 degree C. It is recommended to not freeze. Bring product to room temperature prior to using.

tested application :

Block, EIA/ELISA, Flow Cytometry, Immunohistochemistry - Formalin, Western Blot

app notes :

Suitable for use in Flow Cytometry (1:15-1:30). Use 20 ul working dilution per/5x10^5 cells/test or 100 ul blood sample. Each laboratory should determined an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

other info1 :

Immunogen: Human thymocytes. Affinity Constant: Not determined

other info2 :

Buffer: Lyophilized from PBS containing 1mg/ml BSA. Preservative: No. Lyophilized: Yes

products categories :

Monoclonal Antibodies to Human CDs

ncbi gi num :

3947700

ncbi acc num :

CAA60883.1

uniprot acc num :

Q13969

ncbi pathways :

Adaptive Immunity Signaling Pathway (366160); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Arf1 Pathway (137927); Binding And Entry Of HIV Virion Pathway (105986); C-MYB Transcription Factor Network Pathway (138073); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (119552)

ncbi summary :

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq]

uniprot summary :

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. Protein type: Cell surface; Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; external side of plasma membrane; lipid raft. Molecular Function: protein binding; enzyme binding; protein homodimerization activity; transmembrane receptor activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding. Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; signal transduction; induction by virus of cell-cell fusion in host; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation. Disease: Okt4 Epitope Deficiency

size1 :

0.2 mg

price1 :

405 USD

more info or order :

MyBioSource product webpage