catalog number :
MBS311979
products full name :
MAb to CD35
products short name :
CD35
products name syn :
Monoclonal Antibody to CD35, C3b Receptor 160, 190, 220, and 250kDa
specificity :
CD35. CD35 antigen is composed of 4 allotypes. C3b Receptor (CR1) is present on B lymphocytes, monocytes, myeloid cells, dendritic cells of lymphocyte follicules and renal podocytes.
form :
Purified, Lyophilized. Reconstitute with 1ml distilled water.
concentration :
0.2 mg/ml (prior to lyophilization)
storage stability :
Store lyophilized product at 2 to 8 degree C. After reconstitution, aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles. The addition of 0.1% sodium azide is recommended for storage up to one month at 2 to 8 degree C.
tested application :
Block, EIA/ELISA, Flow Cytometry, Immunohistochemistry - Formalin/Paraffin. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necess
app notes :
Mechanism of degradation of C3b. Analysis of lymphoid and myeloid differentiation. J3D3 is a blocking antibody. It inhibits CR1 mediated decay of cells and blocks C3b dependent rosette formation with lymphocytes. Fluorescence microscopy or flow cytometry: 2ug/5x10^5 cells/tests. Immunohistochemistry: Working dilution: 1:25 to 1:50. Suitable on frozen sections or cell smears and on Bouin's fixed, paraffin-embedded tissues.
other info1 :
Volume: 1ml. Immunogen: Purified C3b receptor from human red blood cells. Affinity Constant: Not determined
other info2 :
Buffer: Lyophilized from PBS containing 1mg/ml BSA. Preservative: No. Lyophilized: Yes. Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
products categories :
Monoclonal Antibodies to Human CDs
products references :
Cook J., et al., (1985), "Mouse monoclonal antibodies to the human C3b receptor", Molec. Immunol., 22 #5, 531-539. Reid, K.B.M., "Complement system protein which interact with C3b or C4d", Immunology Today, 7, 7