MAb to Beta d Galactosidase (E. coli) | MyBioSource MBS311379 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

MAb to Beta d Galactosidase (E. coli)

catalog :

MBS311379

quantity :

1 mg

price :

310 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

2.00E+09

reactivity :

Escherichia coli str. K-12 substr. MG1655

product information

catalog number :

MBS311379

products type :

Antibody

products full name :

MAb to Beta d Galactosidase (E. coli)

products short name :

Beta d Galactosidase (E. coli)

products name syn :

MAb to Beta d Galactosidase (E.coli); Monoclonal Antibody to Beta d Galactosidase (E. coli)

other names :

beta-D-galactosidase; Beta-galactosidase; beta-D-galactosidase; Lactase

other gene names :

lacZ; lacZ; ECK0341; JW0335

uniprot entry name :

BGAL_ECOLI

clonality :

Monoclonal

isotype :

IgG1

clone :

2.00E+09

host :

Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.

sequence length :

1024

specificity :

Beta d Galactosidase (E. coli). B-galactosidase of E. coli and Aspergillus oryzae. Cross-reactivity with B-galactosidase from bovine liver <10%. Clone 2E9 does not influence enzyme activity.

purity :

>90% pure. Protein A Sepharose chromatography. Purity is tested by electrophoresis.

form :

Purified, Liquid

concentration :

1.9mg/ml (OD280nm, E^0.1% = 1.4)

storage stability :

Store at 2 to 8 degree C.

app notes :

Detection of beta-galactosidase expressed by LacZ gene in cloned sequences. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessar

other info1 :

Immunogen: Purified beta-galactosidase of E. coli. Affinity Constant: Not determined.

other info2 :

Buffer: PBS, pH 7.4. Preservative: 0.1% Sodium Azide. Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

products categories :

Monoclonal Antibodies to Enzymes

ncbi gi num :

16128329

ncbi acc num :

NP_414878.1

ncbi gb acc num :

NC_000913.2

uniprot acc num :

P00722

ncbi mol weight :

116,483 Da

ncbi pathways :

Galactose Metabolism Pathway 1012!!Galactose Metabolism Pathway 292!!Metabolic Pathways 131985!!Other Glycan Degradation Pathway 1052!!Other Glycan Degradation Pathway 346!!Sphingolipid Metabolism Pathway 1065!!Sphingolipid Metabolism Pathway 369!!Lactose Degradation III Pathway 192!!Lactose Degradation III Pathway 139429

ncbi summary :

Repressed during biofilm formation. [More information is available at EcoGene: EG10527]. galacotsidase is a metalloenzyme exhibiting broad substrate specificity. [More information is available at EcoCyc: EG10527].

uniprot summary :

Catalytic activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687. Cofactor: Binds 2 magnesium ions per monomer. Can also use manganese. Ref.9 Ref.13 Ref.20Binds 1 sodium ion per monomer. Ref.9 Ref.13 Ref.20. Enzyme regulation: Inhibited by phenylethyl thio-beta-D-galactoside (PETG), isopropyl thio-beta-D-galactoside (IPTG), L-ribose, D-galactonolactone, lactose and 2-amino-D-galactose. Ref.24. Subunit structure: Homotetramer. Ref.21. Induction: By allolactose. Ref.8 Ref.24. Sequence similarities: Belongs to the glycosyl hydrolase 2 family. Biophysicochemical propertiesKinetic parameters:The values for the enzymatic assays using manganese as cofactor are very close.KM=0.04 mM for p-nitrophenyl beta-D-galactoside Ref.9 Ref.14 Ref.15 Ref.17 Ref.18 Ref.24KM=0.12 mM for o-nitrophenyl beta-D-galactosideKM=0.15 mM for 2,3-dinitrophenyl beta-D-galactopyranosideKM=0.41 mM for 2,5-dinitrophenyl beta-D-galactopyranosideKM=11.6 mM for p-nitrophenol-alpha-L-arabinopyranosideKM=16.9 mM for p-nitrophenol-beta-D-fucopyranosideKM=34 uM for p-nitrophenyl beta-D-galactoside (with magnesium as cofactor and 30 degrees Celsius)KM=140 uM for o-nitrophenyl beta-D-galactoside (with magnesium as cofactor and 30 degrees Celsius)KM=940 uM for allolactose (with magnesium as cofactor and 30 degrees Celsius)KM=1350 uM for lactose (with magnesium as cofactor and 30 degrees Celsius)Vmax=30.9 umol/min/mg enzyme with lactose as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=49.7 umol/min/mg enzyme with allolactose as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=59.7 umol/min/mg enzyme with p-nitrophenyl beta-D-galactoside as substrate (with magnesium as cofactor and 30 degrees Celsius)Vmax=360 umol/min/mg enzyme with o-nitrophenyl beta-D-galactoside as substrate (with magnesium as cofactor and 30 degrees Celsius)

size :

1 mg

price :

310 USD