MAb to Major Basic Protein | MyBioSource MBS311297 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MAb to Major Basic Protein

catalog :

MBS311297

quantity :

0.1 mg

price :

315 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BMK13

reactivity :

human

application :

western blot, ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS311297

products type :

Antibody

products full name :

MAb to Major Basic Protein

products short name :

Major Basic Protein (Eosinophil Marker)

products name syn :

Monoclonal Antibody to Human Eosinophil Major Basic Protein (MBP)

other names :

major basic protein; Bone marrow proteoglycan; bone marrow proteoglycan; OTTHUMP00000234774; OTTHUMP00000234775; OTTHUMP00000234776; bone-marrow proteoglycan; proteoglycan 2 preproprotein; natural killer cell activator; eosinophil major basic protein; eosinophil granule major basic protein; proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein); Proteoglycan 2Cleaved into the following chain:Eosinophil granule major basic protein; EMBP; MBP; Alternative name(s):; Pregnancy-associated major basic protein

other gene names :

PRG2; PRG2; MBP; BMPG; MBP1; MGC14537; MBP

uniprot entry name :

PRG2_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

BMK13

host :

Host: Mouse. Source: Tissue Culture Supernatant

specificity :

Major Basic Protein (Eosinophil Marker). Specific for eosinophil major basic protein irrespective of the stages of eosinophil activation. Recognizes the 23.8kDa secreted pro-form of MBP (Pregnancy associated MBP) and the 13.8kDa mature form found in the

purity :

Protein A chromatography

form :

Purified, Liquid

concentration :

100ug/ml

storage stability :

Short-term (up to 1 month) store at 2 to 8 degree C. Long term, aliquot and store at -20 degree C. Avoid multiple/freeze thaw cycles.

tested application :

EIA/ELISA, Immunohistochemistry - Paraffin, Western Blot

app notes :

Suitable for use in IHC with fresh frozen tissue (fix in methanol:acetone), IHC with formalin fixed paraffin-embedded tissue (treat with 0.1% trypsin before blocking), Western Blot and ELISA.

other info1 :

Affinity Constant: Not determined

other info2 :

Buffer: PBS containing 0.1% BSA. Preservative: 0.1%Sodium azide. Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

products categories :

Monoclonal Antibodies to Proteins and Bioactive Peptides

products references :

Bernardes, J.F., et al., (2004), "Protein nitration in chronic sinusitis and nasal polyposis: role of eosinophils", Otolaryngol. Head and Neck Surg., 131(5): 696-703. Ying, S., et al., (2002), "Methods for identifying human eosin

ncbi gi num :

187415

uniprot acc num :

P13727

ncbi mol weight :

25,206 Da

ncbi pathways :

Asthma Pathway (83120); Asthma Pathway (532)

ncbi summary :

The protein encoded by this gene is the predominant constituent of the crystalline core of the eosinophil granule. High levels of the proform of this protein are also present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein may be involved in antiparasitic defense mechanisms as a cytotoxin and helminthotoxin, and in immune hypersensitivity reactions. It is directly implicated in epithelial cell damage, exfoliation, and bronchospasm in allergic diseases. [provided by RefSeq]

uniprot summary :

Function: Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. Ref.22. Subunit structure: In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg. Ref.11 Ref.12 Ref.16 Ref.19 Ref.22 Ref.23. Subcellular location: Bone marrow proteoglycan: Secreted. Note: The proform is secreted.Eosinophil granule major basic protein: Cytoplasmic vesicle secretory vesicle. Note: The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule (crystalloid core). Tissue specificity: High levels of the proform in placenta and pregnancy serum; in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon. Ref.20 Ref.21. Developmental stage: Levels of the proform increase in serum and placenta during pregnancy. Ref.12 Ref.21. Post-translational modification: Nitrated. Miscellaneous: Binds heparin. Does not bind calcium. Sequence similarities: Contains 1 C-type lectin domain.

size1 :

0.1 mg

price1 :

315 USD

more info or order :

MyBioSource product webpage