MAb to glyceraldehyde-3-PDH | MyBioSource MBS311207 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

MAb to glyceraldehyde-3-PDH

catalog :

MBS311207

quantity :

1 mg

price :

1040 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS311207

products type :

Antibody

products full name :

MAb to glyceraldehyde-3-PDH

products short name :

Glyceraldehyde-3-Phosphate Dehydrogenase (multiple species) (GAPDH)

products name syn :

Monoclonal Antibody to Glyceraldehyde-3-PDH (GAPDH)

other names :

glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; OTTHUMP00000174430; OTTHUMP00000174431; OTTHUMP00000174432; OTTHUMP00000174434; aging-associated gene 9 protein; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH

other gene names :

GAPDH; GAPDH; G3PD; GAPD; MGC88685; GAPD

uniprot entry name :

G3P_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

6C5

host :

Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.

sequence length :

335

specificity :

Glyceraldehyde-3-Phosphate Dehydrogenase (multiple species) (GAPDH). Recognizes GAPDH monomer (36 kDa) ad the dimer form. Does not react with the tetrameric form. Reacts with GAPDH from fish, frog, chicken, rabbit, mouse and human skeletal muscle. Does

purity :

>90% pure. Protein A Sepharose chromatography. Purity is tested by electrophoresis.

form :

HRP, Liquid

concentration :

0.9mg/ml (Sigma protein assay)

storage stability :

Store at 2 to 8 degree C.

app notes :

Detection of GAPDH. Immunoaffinity purification of GAPDH. Works in ELISA and Western blotting. Works in Immunofluorescence and Immunocytochemistry. Each laboratory should determine an optimum working titer for use in its particular application

other info1 :

Immunogen: Purified rabbit muscle GAPDH (whole molecule).

other info2 :

Buffer: PBS, pH 7.4. Preservative: 0.05% Proclin. Warnings: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

products categories :

Monoclonal Antibodies to Enzymes

ncbi gi num :

7669492

ncbi acc num :

NP_002037.2

ncbi gb acc num :

NM_002046.3

uniprot acc num :

P04406

ncbi mol weight :

36,053 Da

ncbi pathways :

Alzheimer's Disease Pathway 83097!!Alzheimer's Disease Pathway 509!!Androgen Receptor Signaling Pathway 198806!!Gluconeogenesis Pathway 106204!!Glucose Metabolism Pathway 106199!!Glycolysis Pathway 105911!!Glycolysis / Gluconeogenesis Pathway 82926!!Glycolysis / Gluconeogenesis Pathway 287!!Metabolic Pathways 132956!!Metabolism Of Carbohydrates Pathway 106196

ncbi summary :

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

uniprot summary :

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Ref.6 Ref.23. Catalytic activity: D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH. Ref.6. Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. Subunit structure: Homotetramer. Interacts with TPPP; the interaction is direct. Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. By similarity. Interacts with EIF1AD, USP25, PRKCI and WARS. Ref.23 Ref.25 Ref.28 Ref.36 Ref.39 Ref.40. Subcellular location: Cytoplasm cytosol. Nucleus. By similarity. Cytoplasm perinuclear region. Membrane. Note: Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. By similarity. Postnuclear and Perinuclear regions. Ref.24. Post-translational modification: S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. By similarity.ISGylated. Probable. Ref.29. Sequence similarities: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

size :

1 mg

price :

1040 USD

more info or order :

MyBioSource product webpage