MAb to Proinsulin | MyBioSource MBS310707 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

MAb to Proinsulin

catalog :

MBS310707

quantity :

1 mg

price :

420 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

enzyme immunoassay, ELISA, immunohistochemistry, radioimmunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS310707

products type :

Antibody

products full name :

MAb to Proinsulin

products short name :

Proinsulin

products name syn :

Monoclonal Antibody to Human Proinsulin

other names :

insulin preproprotein; Insulin; insulin; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519; insulin; N/A

other gene names :

INS; INS; ILPR; IRDN; IDDM2; MODY10; N/A

uniprot entry name :

INS_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

3A1

host :

Mouse. Hybridization of X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice.

sequence length :

110

specificity :

Proinsulin. Reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule. Does not cross-react with human, bovine, porcine insulin, bovine and porcine proinsulin, and human C-peptide.

purity :

>90% pure (SDS-PAGE). Protein A chromatography

form :

Purified, Liquid

concentration :

5.7mg/ml (OD280nm, E^0.1% = 1.4)

storage stability :

Store at 2 to 8 degree C.

tested application :

EIA/ELISA, Immunohistochemistry, Immunoassay Antibody Pairs, Radioimmunoassay

app notes :

Detection of human proinsulin in two-site enzyme immunoassay. Can be used in immunohistochemistry on frozen sections. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested

other info1 :

Immunogen: Purified human proinsulin. Affinity Constant: 1.1 x 10^-8 M

other info2 :

Buffer: PBS, pH 7.4. Preservative: 0.1% Sodium azide. Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

products categories :

Monoclonal Antibodies to Hormones and Steroids

ncbi gi num :

4557671

ncbi acc num :

NP_000198.1

ncbi gb acc num :

NM_000207.2

uniprot acc num :

P01308

ncbi mol weight :

11,981 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway 138006!!Adipogenesis Pathway 198832!!Aldosterone-regulated Sodium Reabsorption Pathway 130626!!Aldosterone-regulated Sodium Reabsorption Pathway 130590!!Amyloids Pathway 366238!!Arf6 Trafficking Events Pathway 137954!!Diabetes Pathways 105902!!Downstream Signaling Of Activated FGFR Pathway 160957!!FOXA1 Transcription Factor Network Pathway 137979!!FOXA2 And FOXA3 Transcription Factor Networks Pathway 137911

ncbi summary :

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

uniprot summary :

Function: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Subunit structure: Heterodimer of a B chain and an A chain linked by two disulfide bonds. Subcellular location: Secreted. Involvement in disease: Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [. MIM:176730]. Ref.21 Ref.23 Ref.24 Ref.25Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [. MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Ref.34Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [. MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy. Ref.32 Ref.33Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [. MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. Ref.33 Ref.34 Ref.35. Pharmaceutical use: Available under the names Humulin or Humalog (Eli Lilly) and Novolin (Novo Nordisk). Used in the treatment of diabetes. Humalog is an insulin analog with 52-Lys-Pro-53 instead of 52-Pro-Lys-53. Sequence similarities: Belongs to the insulin family. Sequence caution: The sequence AAA59179.1 differs from that shown. Reason: Erroneous gene model prediction.

size :

1 mg

price :

420 USD

more info or order :

MyBioSource product webpage