antibody

MAb to Insulin

MBS310686

1 mg

465 USD

monoclonal

mouse

nonconjugated

7F8

ELISA, radioimmunoassay, enzyme immunoassay

Antibody

MAb to Insulin

Insulin

Monoclonal Antibody to Human Insulin

insulin; Insulin; insulin; proinsulin; OTTHUMP00000011161; OTTHUMP00000011162; OTTHUMP00000196036; OTTHUMP00000196038; OTTHUMP00000217519; insulin

INS; INS; ILPR; IRDN; IDDM2; MODY10

INS_HUMAN

Monoclonal

IgG1, kappa

7F8

Mouse. Hybridization of X63-Ag8-653 myeloma cells with spleen cells from BALB/c mice.

110

Insulin, human. Cross-reacts with human proinsulin, bovine insulin (30%) and porcine insulin. Does not react with free C-peptide.

>90% pure (SDS-PAGE). Protein A chromatography

Purified, Liquid

8.1 mg/ml (OD280nm, E^0.1% = 1.4)

Store at 2 to 8 degree C.

EIA/ELISA, Immunoassay Antibody Pairs, Radioimmunoassay

Detection of insulin in two-site enzyme immunoassay. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Capture: . MBS310686. MBS310707. Detection: . MBS310703. MBS310686 (Insulin)

Immunogen: Purified human insulin. Affinity Constant: Kass=7.5 x 10^9M^-1

Buffer: PBS, pH 7.4. Preservative: 0.1% Sodium azide. Important Note: Centrifuge before opening to ensure complete recovery of vial contents. Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

Monoclonal Antibodies to Hormones and Steroids

386828

AAA59172.1

P01308

11,981 Da

ATF-2 Transcription Factor Network Pathway (138006); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Amyloids Pathway (366238); Arf6 Trafficking Events Pathway (137954); Diabetes Pathways (105902); Downstream Signaling Of Activated FGFR Pathway (160957); FOXA1 Transcription Factor Network Pathway (137979); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911)

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family. Protein type: Secreted; Secreted, signal peptide; Hormone. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; endoplasmic reticulum lumen; Golgi lumen; extracellular region. Molecular Function: insulin-like growth factor receptor binding; identical protein binding; protein binding; protease binding; hormone activity; insulin receptor binding. Biological Process: positive regulation of nitric oxide biosynthetic process; glucose homeostasis; positive regulation of vasodilation; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; positive regulation of nitric-oxide synthase activity; activation of protein kinase B; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of cell differentiation; negative regulation of protein catabolic process; regulation of amino acid metabolic process; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; positive regulation of MAPKKK cascade; negative regulation of vasodilation; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; alpha-beta T cell activation; negative regulation of fatty acid metabolic process; endocrine pancreas development; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; energy reserve metabolic process; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration. Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10

1 mg

465 USD