Mouse Anti-Human Epidermal Growth Factor Receptor (EGFR) Monoclonal Antibody (Clone SPM341) | MyBioSource MBS302895 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human Epidermal Growth Factor Receptor (EGFR) Monoclonal Antibody (Clone SPM341)

catalog :

MBS302895

quantity :

0.5 ml

price :

400 USD

clonality :

monoclonal

host :

mouse

reactivity :

rat, human, mouse

application :

immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS302895

products type :

Antibody

products full name :

Mouse Anti-Human Epidermal Growth Factor Receptor (EGFR) Monoclonal Antibody (Clone SPM341)

products short name :

Epidermal Growth Factor Receptor

other names :

epidermal growth factor receptor isoform a; Epidermal growth factor receptor; epidermal growth factor receptor; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

products gene name :

EGFR

other gene names :

EGFR; EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; ERBB; ERBB1; HER1

uniprot entry name :

EGFR_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

SPM341

host :

Mouse

reactivity :

Human. Predicted Reactivity: Bovine, Mouse, Pig, Rat

sequence length :

1210

form :

0.5 ml mouse monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.primary antibody. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:25. The Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:25. The Antigen Retrieval: Digest tissue sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37 degree C. Primary Antibody Incubation: Incubate for 30 min at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:25. IHC Positive Control: Skin, Placenta, Squamous Cell Carcinoma

other info2 :

Immunogen: Extracellular domain of human recombinant EGFR protein. Control Tissue: Placenta, Skin, Squamous Cell Carcinoma. Cellular Localization: Membrane. Entrez Gene Code: 1956. Pathway: EGFR Family

products description :

EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-alpha (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

ncbi gi num :

29725609

ncbi acc num :

NP_005219.2

ncbi gb acc num :

NM_005228.3

ncbi mol weight :

170 kDa (Wild Type) and 145 kDa (VIII Variant)

ncbi pathways :

AGE/RAGE Pathway 698754!!Adaptive Immune System Pathway 366160!!Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!AhR Pathway 755436!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Androgen Receptor Signaling Pathway 198806!!Arf6 Signaling Events Pathway 138034!!Axon Guidance Pathway 105688!!Bladder Cancer Pathway 83115

ncbi summary :

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

size :

0.5 ml

price :

400 USD

more info or order :

MyBioSource product webpage