product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human CD8 Monoclonal Antibody (Clone SP16)
catalog :
MBS302863
quantity :
1 ml
price :
600 USD
clonality :
monoclonal
host :
rabbit
reactivity :
human
application :
immunohistochemistry - paraffin section, immunohistochemistry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS302863
products type :
Antibody
products full name :
Rabbit Anti-Human CD8 Monoclonal Antibody (Clone SP16)
products short name :
CD8
other names :
T-cell surface glycoprotein CD8 alpha chain isoform 1; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; CD8 antigen, alpha polypeptide (p32); Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T-cell antigen Leu2; T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2; CD_antigen: CD8a
products gene name :
CD8
other gene names :
CD8A; CD8A; CD8; MAL; p32; Leu2; MAL
uniprot entry name :
CD8A_HUMAN
clonality :
Monoclonal
isotype :
IgG
clone :
SP16
host :
Rabbit
reactivity :
Human
sequence length :
235
form :
1.0 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Tonsil
other info2 :
Immunogen: Synthetic peptide corresponding to C-terminus of alpha chain of the human CD8 molecule. Control Tissue: Tonsil. Cellular Localization: Membrane. Entrez Gene Code: 925. Pathway: Hematopoiesis
products description :
CD8 molecule consists of two chains, termed alpha and beta chain, which are expressed as a disulphide-linked alpha/beta heterdimer or as an alpha/alpha homodimer on T cell subset, thymocytes and NK cells. The majority of CD8+ T cells express CD8 as alpha/beta heterdimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 alpha chain (but not beta chain).
ncbi gi num :
225007536
ncbi acc num :
NP_001139345.1
ncbi gb acc num :
NM_001145873.1
ncbi mol weight :
32 kDa
ncbi pathways :
Adaptive Immune System Pathway 366160!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Downstream Signaling In Naive CD8+ T Cells Pathway 138018!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!IL12-mediated Signaling Events Pathway 137922!!Immune System Pathway 106386
ncbi summary :
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
size :
1 ml
price :
600 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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