Rabbit Anti-Human MCM2 Monoclonal Antibody (Clone SP85) | MyBioSource MBS302835 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human MCM2 Monoclonal Antibody (Clone SP85)

catalog :

MBS302835

quantity :

0.1 ml

price :

265 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

SP85

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

product information

catalog number :

MBS302835

products type :

Antibody

products full name :

Rabbit Anti-Human MCM2 Monoclonal Antibody (Clone SP85)

products short name :

MCM2

other names :

DNA replication licensing factor MCM2; DNA replication licensing factor MCM2; DNA replication licensing factor MCM2; MCM2 minichromosome maintenance deficient 2, mitotin; cell devision cycle-like 1; cyclin-like 1; minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance protein 2 homolog; nuclear protein BM28; minichromosome maintenance complex component 2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28

products gene name :

MCM2

other gene names :

MCM2; MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN; BM28; CCNL1; CDCL1; KIAA0030

uniprot entry name :

MCM2_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP85

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog, Frog, Mouse, Rat

sequence length :

904

form :

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. dilutions are estimates; actual results may differ because of variability in methods and protocols. for 20 min. for 20 min. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Rectal Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: HeLa Cell Lysate. Flow Cytometry . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: HeLa Cell Line

other info2 :

Immunogen: Synthetic peptide derived from internal region of human MCM2 protein. Control Tissue: Rectal Carcinoma. Cellular Localization: Nucleus. Entrez Gene Code: 4171. Pathway: Cell Cycle

products description :

MCM2 (Mini chromosome maintenance protein 2) is involved in regulating DNA replication. MCM2 (also called CDCL1, mitotin and BM28), is a human nuclear protein that is crucial in the cell cycle, being involved in the onset of DNA replication and cell division. It is similar to members of the family of early S-phase proteins. Mincheva et al. (1994) mapped the gene to 3q21. From its localization, CDCL1 became a candidate for an oncogene affected by chromosomal breaks in acute myeloid leukemia (AML).

ncbi gi num :

33356547

ncbi acc num :

NP_004517.2

ncbi gb acc num :

NM_004526.3

ncbi mol weight :

130 kDa

ncbi pathways :

Activation Of ATR In Response To Replication Stress Pathway 105754!!Activation Of The Pre-replicative Complex Pathway 105777!!Assembly Of The Pre-replicative Complex Pathway 105821!!Cell Cycle Pathway 530733!!Cell Cycle Checkpoints Pathway 105739!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054!!Cell Cycle Pathway 463!!DNA Replication Pathway 198771

ncbi summary :

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]

size :

0.1 ml

price :

265 USD