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antibody

Rabbit Anti-Human GFAP Monoclonal Antibody (Clone SP78)

MBS302831

1 ml

705 USD

monoclonal

rabbit

human, mouse, dog, rat

immunohistochemistry - paraffin section, immunohistochemistry

MBS302831

Antibody

Rabbit Anti-Human GFAP Monoclonal Antibody (Clone SP78)

Glial Fibrillary Acidic Protein

glial fibrillary acidic protein isoform 2; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein; N/A

GFAP

GFAP; GFAP; GFAP

GFAP_HUMAN

Monoclonal

IgG

SP78

Rabbit

Human. Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rabbit, Rat

431

1.0 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Astrocytoma

Immunogen: Synthetic peptide corresponding to C-terminus of human GFAP protein. Control Tissue: Astrocytoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 2670. Pathway: Stem Cell

Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition, many types of brain tumors presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

196115290

NP_001124491.1

NM_001131019.2

51-52 kDa

Neural Crest Differentiation Pathway 672460!!Nuclear Signaling By ERBB4 Pathway 530744!!Signal Transduction Pathway 477114!!Signaling By ERBB4 Pathway 530741!!Spinal Cord Injury Pathway 739007

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

1 ml

705 USD