Rabbit Anti-Human Bax Monoclonal Antibody (Clone SP47) | MyBioSource MBS302765 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Bax Monoclonal Antibody (Clone SP47)

catalog :

MBS302765

quantity :

0.1 ml

price :

265 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

SP47

reactivity :

human, mouse, rat

application :

immunohistochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

product information

catalog number :

MBS302765

products type :

Antibody

products full name :

Rabbit Anti-Human Bax Monoclonal Antibody (Clone SP47)

products short name :

BAX

other names :

apoptosis regulator BAX isoform beta; Apoptosis regulator BAX; apoptosis regulator BAX; BCL2-associated X protein omega; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; bcl-2-like protein 4; bcl2-L-4; BCL2-associated X protein; Bcl-2-like protein 4; Bcl2-L-4

products gene name :

BAX

other gene names :

BAX; BAX; BCL2L4; BCL2L4; Bcl2-L-4

uniprot entry name :

BAX_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP47

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Mouse, Rat

sequence length :

218

form :

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilutions: If using the concentrate format of this product, dilute the antibody 1:50 prior to use. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Primary Antibody: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Normal Breast. Flow Cytometry . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: HeLa Cell Line

other info2 :

Immunogen: Synthetic peptide corresponding to internal region of human BAX protein. Control Tissue: Breast, Colon. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 581. Pathway: Apoptosis

products description :

Bax is a member of the Bcl-2 family of proteins, which function as regulators of apoptosis.Overexpression of Bax functions to promote cell death. Bax can form homodimers and is also ableto heterodimerize with other Bcl-2 related proteins.

ncbi gi num :

4757838

ncbi acc num :

NP_004315.1

ncbi gb acc num :

NM_004324.3

ncbi mol weight :

21 kDa

ncbi pathways :

Activation, Translocation And Oligomerization Of BAX Pathway 105665!!AhR Pathway 755436!!Amyotrophic Lateral Sclerosis (ALS) Pathway 920975!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83099!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 198797!!Apoptosis Pathway 83060!!Apoptosis Pathway 470!!Apoptosis Pathway 105648!!Apoptosis Modulation And Signaling Pathway 198822

ncbi summary :

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

size :

0.1 ml

price :

265 USD