Rabbit Anti-Human Glypican-3 Polyclonal Antibody | MyBioSource MBS302738 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Glypican-3 Polyclonal Antibody

catalog :

MBS302738

quantity :

0.5 ml

price :

475 USD

clonality :

monoclonal

host :

rabbit

reactivity :

human

application :

flow cytometry, western blot, immunohistochemistry, FACS, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS302738

products type :

Antibody

products full name :

Rabbit Anti-Human Glypican-3 Polyclonal Antibody

products short name :

Glypican-3

other names :

glypican-3 isoform 1; Glypican-3; glypican-3; glypican proteoglycan 3; heparan sulphate proteoglycan; intestinal protein OCI-5; secreted glypican-3; glypican 3; GTR2-2; Intestinal protein OCI-5; MXR7Secreted glypican-3

other gene names :

GPC3; GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; OCI5

uniprot entry name :

GPC3_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP86

host :

Rabbit

reactivity :

Human

sequence length :

603

form :

0.5 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Placenta, Liver Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: HepG2 Cell Lysate. Flow Cytometry . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: HEPG2 Cell Line

other info2 :

Immunogen: Synthetic peptide derived from C-terminus of human glypican-3 protein. Control Tissue: Placenta. Cellular Localization: Membrane. Entrez Gene Code: 2719. Pathway: Signal Transduction

products description :

Glypican-3 (GPC3) is a cell surface proteoglycan that bears heparan sulfate. It may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. The expression of GPC3 is relatively high in liver, placenta, lung, and kidney. Alteration of GPC3 expression is associated with several malignancies such as hepatocellular carcinoma (HCC). This antibody can be used to detect GPC3 in both normal and neoplastic tissue types. This antibody should not be used to differentiate hepatocellular carcinoma (HCC) from either normal liver or other types of liver disease.

ncbi gi num :

257471006

ncbi acc num :

NP_001158089.1

ncbi gb acc num :

NM_001164617.1

ncbi mol weight :

64 kDa

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway 645305!!Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway 645308!!Defective B3GAT3 Causes JDSSDHD Pathway 1127588!!Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway 1127592!!Defective B4GALT7 Causes EDS, Progeroid Type Pathway 1127587!!Defective CHST14 Causes EDS, Musculocontractural Type Pathway 1127591!!Defective CHST3 Causes SEDCJD Pathway 1127590!!Defective CHST6 Causes MCDC1 Pathway 1127593!!Defective CHSY1 Causes TPBS Pathway 1127589!!Defective EXT1 Causes Exostoses 1, TRPS2 And CHDS Pathway 1127594

ncbi summary :

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

size :

0.5 ml

price :

475 USD

more info or order :

MyBioSource product webpage