MyBioSource

antibody

Rabbit Anti-Human Ku80 Monoclonal Antibody (Clone SP130)

MBS302722

7 ml

425 USD

monoclonal

rabbit

rat, human, mouse

western blot, immunohistochemistry - paraffin section, immunohistochemistry

MBS302722

Antibody

Rabbit Anti-Human Ku80 Monoclonal Antibody (Clone SP130)

Ku80

X-ray repair cross-complementing protein 5; X-ray repair cross-complementing protein 5; X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen, 80kDa; Ku86 autoantigen related protein 1; TLAA; lupus Ku autoantigen protein p86; nuclear factor IV; thyroid-lupus autoantigen; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)

Ku80

XRCC5; XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1; G22P2; CTC85; CTCBF; TLAA

XRCC5_HUMAN

Monoclonal

IgG

SP130

Rabbit

Human. Predicted Reactivity: Bovine, Mouse, Rat

732

7.0 ml pre-diluted rabbit monoclonal antibody purified by protein A/G in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin, Western Blotting (WB)

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Tonsil. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: HeLa Cell Lysate

Immunogen: Synthetic peptide corresponding to an internal region of human Ku80 protein. Control Tissue: Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 7520. Pathway: DNA Replication and Repair

X-ray repair cross-complementing protein 5 (XRCC5), alternative name (Ku80), is single stranded DNA-dependent ATP-dependent helicase. It involves in chromosome translocation. Ku80 along with Ku70 forms a heterodimeric protein originally identified as autoantigens associated with several autoimmune diseases including scleroderma, polymyositis and systemic lupus erythematosus. Ku is widely expressed nuclear protein that binds to and stabilizes the ends of DNA at telomeres or double-stranded DNA breaks. The inhibition of Ku80 can damage the DNA repair pathway of tumor cells and potentially increase the killing of cancer cells, and reduce drug resistance.

10863945

NP_066964.1

NM_021141.3

86 kDa

2-LTR Circle Formation Pathway 105993!!BARD1 Signaling Events Pathway 137959!!Coregulation Of Androgen Receptor Activity Pathway 138085!!Cytosolic Sensors Of Pathogen-associated DNA Pathway 576255!!DNA Repair Pathway 105837!!DNA-PK Complex Pathway 413430!!DNA-PK Complex Pathway 890556!!Disease Pathway 530764!!Double-Strand Break Repair Pathway 105861!!Early Phase Of HIV Life Cycle Pathway 105985

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

7 ml

425 USD