Rabbit Anti-Human Keratin 18 Monoclonal Antibody (Clone SP69) | MyBioSource MBS302710 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Keratin 18 Monoclonal Antibody (Clone SP69)

catalog :

MBS302710

quantity :

0.5 ml

price :

485 USD

clonality :

monoclonal

host :

rabbit

reactivity :

mouse, human, rat

application :

flow cytometry, western blot, FACS, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS302710

products type :

Antibody

products full name :

Rabbit Anti-Human Keratin 18 Monoclonal Antibody (Clone SP69)

products short name :

Keratin 18

other names :

keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 18; keratin, type I cytoskeletal 18; CK-18; cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; cytokeratin 18; cytokeratin-18; keratin 18, type I; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

other gene names :

KRT18; KRT18; K18; CYK18; CYK18; CK-18; K18

uniprot entry name :

K1C18_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP69

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Frog, Mouse, Rat

sequence length :

430

form :

0.5 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:200. IHC Positive Control: Prostate, Breast Adenoarcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: A431 Cell Lysate. Flow Cytometry . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: HeLa Cell Line

other info2 :

Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 18. Control Tissue: Breast Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 3875. Pathway: Stem Cell

products description :

Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with cytokeratin 8, a basic keratin. Hepatocellular carcinomas express only cytokeratins 8 and 18.

ncbi gi num :

4557888

ncbi acc num :

NP_000215.1

ncbi gb acc num :

NM_000224.2

ncbi mol weight :

48 kDa

ncbi pathways :

Caspase Cascade In Apoptosis Pathway 137974!!EGFR1 Signaling Pathway 198782!!Pathogenic Escherichia Coli Infection Pathway 83103!!Pathogenic Escherichia Coli Infection Pathway 672459!!Pathogenic Escherichia Coli Infection Pathway 516

ncbi summary :

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

size :

0.5 ml

price :

485 USD

more info or order :

MyBioSource product webpage