Rabbit Anti-Human CD4 Monoclonal Antibody (Clone SP35) | MyBioSource MBS302650 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human CD4 Monoclonal Antibody (Clone SP35)

catalog :

MBS302650

quantity :

1 ml

price :

750 USD

clonality :

monoclonal

host :

rabbit

reactivity :

human

application :

immunohistochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

product information

catalog number :

MBS302650

products type :

Antibody

products full name :

Rabbit Anti-Human CD4 Monoclonal Antibody (Clone SP35)

products short name :

CD4

other names :

T-cell surface glycoprotein CD4 isoform 1; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3; CD4 molecule; T-cell surface antigen T4/Leu-3; CD_antigen: CD4

products gene name :

CD4

other gene names :

CD4; CD4; CD4mut

uniprot entry name :

CD4_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP35

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Pig

sequence length :

458

form :

1.0 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Tonsil, T Cell Lymphoma. Flow Cytometry . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: Jurkat cell line

other info2 :

Immunogen: Synthetic peptide corresponding to internal region of human CD4. Control Tissue: T Cell Lymphoma, Tonsil. Cellular Localization: Membrane. Entrez Gene Code: 920. Pathway: Hematopoiesis

products description :

CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV and is expressed in T cell lymphomas. Non-specific nuclear staining is sometimes seen in follicles with this antibody; however in most cases it does not interfere with the interpretation of the CD4 membrane staining.

ncbi gi num :

10835167

ncbi acc num :

NP_000607.1

ncbi gb acc num :

NM_000616.4

ncbi mol weight :

59 kDa

ncbi pathways :

Adaptive Immune System Pathway 366160!!Alpha-defensins Pathway 530758!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Arf1 Pathway 137927!!Binding And Entry Of HIV Virion Pathway 105986!!C-MYB Transcription Factor Network Pathway 138073!!CXCR4-mediated Signaling Events Pathway 137910!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480

ncbi summary :

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

size :

1 ml

price :

750 USD