Rabbit Anti-Human CD31 Monoclonal Antibody (Clone SP164) | MyBioSource MBS302644 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human CD31 Monoclonal Antibody (Clone SP164)

catalog :

MBS302644

quantity :

0.1 ml

price :

265 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

SP164

reactivity :

human

application :

immunohistochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

product information

catalog number :

MBS302644

products type :

Antibody

products full name :

Rabbit Anti-Human CD31 Monoclonal Antibody (Clone SP164)

products short name :

CD31

other names :

platelet endothelial cell adhesion molecule; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule; CD31 antigen; platelet/endothelial cell adhesion molecule 1; EndoCAM; GPIIA'; PECA1; CD_antigen: CD31

products gene name :

CD31

other gene names :

PECAM1; PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM; PECAM-1

uniprot entry name :

PECA1_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP164

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Mouse, Pig

sequence length :

738

form :

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Tonsil. Flow Cytometry . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: Jurkat Cell Line

other info2 :

Immunogen: Synthetic peptide corresponding to C-terminus of mouse CD31. Control Tissue: Tonsil. Cellular Localization: Membrane. Entrez Gene Code: 5175. Pathway: Angiogenesis

products description :

Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31) is a glycoprotein mainly expressed by endothelial cells and platelets. It can be also expressed by some inflammatory cells such as macrophages, granulocytes, NK cells, lymphocytes, and neutrophils.

ncbi gi num :

313760624

ncbi acc num :

NP_000433.4

ncbi gb acc num :

NM_000442.4

ncbi mol weight :

100-130 kDa

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Cell Surface Interactions At The Vascular Wall Pathway 106062!!Extracellular Matrix Organization Pathway 576262!!Hemostasis Pathway 106028!!Integrin Cell Surface Interactions Pathway 106110!!Leukocyte Transendothelial Migration Pathway 83083!!Leukocyte Transendothelial Migration Pathway 494!!Malaria Pathway 152665!!Malaria Pathway 152657

ncbi summary :

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

size :

0.1 ml

price :

265 USD