antibody

Rabbit Anti-Human Bob-1 Monoclonal Antibody (Clone SP92)

MBS302641

0.1 ml

265 USD

monoclonal

rabbit

nonconjugated

SP92

human, cow

western blot, immunohistochemistry, immunohistochemistry - paraffin section

Antibody

Rabbit Anti-Human Bob-1 Monoclonal Antibody (Clone SP92)

BOB-1

POU domain class 2-associating factor 1; POU domain class 2-associating factor 1; POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; POU domain, class 2, associating factor 1; POU class 2 associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1

BOB-1

POU2AF1; POU2AF1; BOB1; OBF1; OCAB; OBF-1; OBF1

OBF1_HUMAN

Monoclonal

IgG

SP92

Rabbit

Human. Predicted Reactivity: Bovine

256

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Tonsil. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Ramos Cell Lysate

Immunogen: Synthetic peptide corresponding to C-terminus of human BOB-1 protein. Control Tissue: Tonsil. Cellular Localization: Nucleus. Entrez Gene Code: 5450. Pathway: Homeodomain Proteins

B cell Oct binding protein 1 (Bob-1), is a transcription factor that is required for germinal center formation and immunoglobulin production. Bob-1 is expressed at high levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell specific activation of Oct-1 and Oct-2.

167900478

NP_006226.2

NM_006235.2

35 kDa

0.1 ml

265 USD