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antibody

Rabbit Anti-Human Actin-beta Monoclonal Antibody (Clone SP124)

MBS302621

1 ml

700 USD

monoclonal

rabbit

human, chicken, mouse, rat, dog

western blot, immunohistochemistry - paraffin section, immunohistochemistry

MBS302621

Antibody

Rabbit Anti-Human Actin-beta Monoclonal Antibody (Clone SP124)

Actin-beta

actin, cytoplasmic 1; Actin, cytoplasmic 1; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1; actin, beta; Beta-actin

ACTB; ACTB; BRWS1; PS1TP5BP1; N/A

ACTB_HUMAN

Monoclonal

Rabbit IgG

SP124

Rabbit

Tested: Human. Expected from sequence similarity: Bovine, Chicken, Dog, Frog, Human, Mouse, Pig, Rabbit, Rat

375

1.0 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. DO NOT FREEZE. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date.

Immunohistochemistry (IHC) (Paraffin), Western Blot (WB)

IHC Dilution: 1:200

Immunogen: Synthetic peptide near the N-terminus of human actin-beta protein. Epitope: Not determined

Cellular Localization: Cytoplasm. Control Tissue: Placenta

Beta-actin is a nonmuscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

4501885

NP_001092.1

NM_001101.3

P60709

42 kDa

Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 117293!!Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway 116129!!Bacterial Invasion Of Epithelial Cells Pathway 149807!!Bacterial Invasion Of Epithelial Cells Pathway 148661!!Chaperonin-mediated Protein Folding Pathway 106242!!Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway 106243!!Dilated Cardiomyopathy Pathway 121494!!Dilated Cardiomyopathy Pathway 121285

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit structure: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Ref.11 Ref.12 Ref.15 Ref.22. Subcellular location: Cytoplasm cytoskeleton. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Ref.17. Post-translational modification: ISGylated. Ref.13Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced . By similarity. Involvement in disease: Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [. MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. Ref.23Defects in ACTB are the cause of Baraitser-Winter syndrome type 1 (BRWS1) [. MIM:243310]. A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. Ref.25. Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Sequence similarities: Belongs to the actin family.

1 ml

700 USD