product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human Keratin 14 Monoclonal Antibody (Clone SP53)
catalog :
MBS302612
quantity :
0.1 ml
price :
265 USD
clonality :
monoclonal
host :
rabbit
reactivity :
rat, mouse, human
application :
FACS, immunohistochemistry, immunohistochemistry - paraffin section, flow cytometry, western blot
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS302612
products type :
Antibody
products full name :
Rabbit Anti-Human Keratin 14 Monoclonal Antibody (Clone SP53)
products short name :
Keratin 14
other names :
keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 14; CK-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); keratin 14, type I; Cytokeratin-14; CK-14; Keratin-14; K14
other gene names :
KRT14; KRT14; K14; NFJ; CK14; EBS3; EBS4; CK-14; K14
uniprot entry name :
K1C14_HUMAN
clonality :
Monoclonal
isotype :
IgG
clone :
SP53
host :
Rabbit
reactivity :
Human. Predicted Reactivity: Bovine, Mouse, Pig, Rat
sequence length :
472
form :
0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilutions: 1:100 Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Primary Antibody: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Skin, Squamous Cell Carcinoma or Prostate. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: A431 Cell Lysate. Flow Cytometry . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: A431 Cell Line
other info2 :
Immunogen: Synthetic peptide corresponding to C-terminus of human cytokeratin 14. Control Tissue: Prostate, Skin, Squamous Cell Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 3861. Pathway: Stem Cell
products description :
Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5.
ncbi gi num :
15431310
ncbi acc num :
NP_000517.2
ncbi gb acc num :
NM_000526.4
ncbi mol weight :
50 kDa
ncbi pathways :
Corticotropin-releasing Hormone Pathway 920957!!Glucocorticoid Receptor Regulatory Network Pathway 138014
ncbi summary :
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
size :
0.1 ml
price :
265 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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