Mouse Anti-Human Cadherin-E/E-Cadherin Monoclonal Antibody (Clone SPM471) | MyBioSource MBS302609 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Mouse Anti-Human Cadherin-E/E-Cadherin Monoclonal Antibody (Clone SPM471)

catalog :

MBS302609

quantity :

0.5 ml

price :

315 USD

clonality :

monoclonal

host :

mouse

reactivity :

human

application :

immunohistochemistry - paraffin section, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS302609

products type :

Antibody

products full name :

Mouse Anti-Human Cadherin-E/E-Cadherin Monoclonal Antibody (Clone SPM471)

products short name :

Cadherin-E/E-Cadherin

other names :

cadherin-1 preproprotein; Cadherin-1; cadherin-1; CAM 120/80; E-Cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; cell-CAM 120/80; epithelial cadherin; uvomorulin; cadherin 1, type 1, E-cadherin (epithelial); CAM 120/80; Epithelial cadherin; E-cadherin; Uvomorulin; CD_antigen: CD324Cleaved into the following 3 chains:E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3

other gene names :

CDH1; CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324; CDHE; UVO; E-cadherin

uniprot entry name :

CADH1_HUMAN

clonality :

Monoclonal

isotype :

IgG2b/k

clone :

SPM471

host :

Mouse

reactivity :

Human

sequence length :

882

form :

0.5 ml mouse monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 30 min at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Breast, Tonsil, Skin

other info2 :

Immunogen: Recombinant protein encoding aa 600-707 of human cadherin-E. Control Tissue: Breast, Skin, Tonsil. Cellular Localization: Membrane. Entrez Gene Code: 999. Pathway: Stem Cell

products description :

E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity.

ncbi gi num :

4757960

ncbi acc num :

NP_004351.1

ncbi gb acc num :

NM_004360.3

ncbi mol weight :

120 kDa

ncbi pathways :

Adaptive Immune System Pathway 366160!!Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Adherens Junctions Interactions Pathway 119533!!Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cell Adhesion Proteins Pathway 105680!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Arf6 Trafficking Events Pathway 137954!!Bacterial Invasion Of Epithelial Cells Pathway 149807

ncbi summary :

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]

size :

0.5 ml

price :

315 USD

more info or order :

MyBioSource product webpage