product summary
request information :
company name :
MyBioSource
product type :
antibody
product name :
Rabbit Anti-Human Alpha-Fetoprotein Monoclonal Antibody (Clone SP154)
catalog :
MBS302585
quantity :
1 ml
price :
705 USD
clonality :
monoclonal
host :
rabbit
reactivity :
dog, human
application :
western blot, immunohistochemistry - paraffin section, immunohistochemistry
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS302585
products type :
Antibody
products full name :
Rabbit Anti-Human Alpha-Fetoprotein Monoclonal Antibody (Clone SP154)
products short name :
Alpha-Fetoprotein
other names :
alpha-fetoprotein; Alpha-fetoprotein; alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin; alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin
products gene name :
AFP
other gene names :
AFP; AFP; AFPD; FETA; HPAFP; HPAFP
uniprot entry name :
FETA_HUMAN
clonality :
Monoclonal
isotype :
IgG
clone :
SP154
host :
Rabbit
reactivity :
Human. Predicted Reactivity: Bovine, Dog, Pig
sequence length :
609
form :
1.0 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Hepatocellular Carcinoma, fetal Liver. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: HepG2 Cell Lysate
other info2 :
Immunogen: Purified human AFP. Control Tissue: Hepatocellular Carcinoma, Fetal Liver. Cellular Localization: Cytoplasm. Entrez Gene Code: 174. Pathway: Stem Cell
products description :
AFP is normally synthesized in fetal liver, intestinal tract, and yolk sac. High level of serum AFP has been identified in patients with hepatocellular carcinomas (HCC), teratoblastoma, colorectal cancer, pancreatic cancer, and germ cell neoplasms.
ncbi gi num :
4501989
ncbi acc num :
NP_001125.1
ncbi gb acc num :
NM_001134.2
ncbi mol weight :
70kDa
ncbi pathways :
Direct P53 Effectors Pathway 137939!!FOXA2 And FOXA3 Transcription Factor Networks Pathway 137911!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!Hippo Signaling Pathway 749777!!Hippo Signaling Pathway 750388
ncbi summary :
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
size :
1 ml
price :
705 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!