Rabbit Anti-Human COX-2 Monoclonal Antibody (Clone SP21) | MyBioSource MBS302577 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human COX-2 Monoclonal Antibody (Clone SP21)

catalog :

MBS302577

quantity :

0.1 ml

price :

265 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

SP21

reactivity :

human, mouse, rat, dog

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS302577

products type :

Antibody

products full name :

Rabbit Anti-Human COX-2 Monoclonal Antibody (Clone SP21)

products short name :

COX-2

other names :

prostaglandin G/H synthase 2; Prostaglandin G/H synthase 2; prostaglandin G/H synthase 2; PGH synthase 2; PHS II; cyclooxygenase 2b; prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase); Cyclooxygenase-2; COX-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; PGHS-2; Prostaglandin-endoperoxide synthase 2

products gene name :

COX-2

other gene names :

PTGS2; PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; COX2; COX-2; PGH synthase 2; PGHS-2

uniprot entry name :

PGH2_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP21

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog, Frog, Mouse, Rat

sequence length :

604

form :

0.1 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Colon Adenoarcinoma, Lung, Breast Carcinoma

other info2 :

Immunogen: Synthetic peptide corresponding to C-terminus of rat COX-2. Control Tissue: Breast Carcinoma, Colon, Lung. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 5743. Pathway: Oncogenesis

products description :

COX2 (Cyclooxygenase-2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate-early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

ncbi gi num :

4506265

ncbi acc num :

NP_000954.1

ncbi gb acc num :

NM_000963.3

ncbi mol weight :

70 kDa

ncbi pathways :

Arachidonic Acid Metabolism Pathway 82991!!Arachidonic Acid Metabolism Pathway 685553!!Arachidonic Acid Metabolism Pathway 366!!C-MYB Transcription Factor Network Pathway 138073!!C20 Prostanoid Biosynthesis Pathway 545299!!C20 Prostanoid Biosynthesis Pathway 908107!!Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway 137993!!Calcium Signaling In The CD4+ TCR Pathway 137941!!Chemical Carcinogenesis Pathway 673221!!Chemical Carcinogenesis Pathway 673237

ncbi summary :

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

size :

0.1 ml

price :

265 USD