Rabbit Anti-Human CD20 Monoclonal Antibody (Clone SP32) | MyBioSource MBS302570 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human CD20 Monoclonal Antibody (Clone SP32)

catalog :

MBS302570

quantity :

1 ml

price :

750 USD

clonality :

monoclonal

host :

rabbit

reactivity :

human, dog

application :

western blot, immunohistochemistry, flow cytometry, FACS, immunohistochemistry - paraffin section

product information

catalog number :

MBS302570

products type :

Antibody

products full name :

Rabbit Anti-Human CD20 Monoclonal Antibody (Clone SP32)

products short name :

CD20

other names :

B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; membrane-spanning 4-domains, subfamily A, member 1; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; CD_antigen: CD20

products gene name :

CD20

other gene names :

MS4A1; MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16; CD20

uniprot entry name :

CD20_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP32

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog

sequence length :

297

form :

1.0 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Tonsil, B Cell Lymphoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Ramos Cell Lysate. Flow Cytometry . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 30 minutes at 4 degree C. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Flow Cytometry Positive Control: Jurkat cell line

other info2 :

Immunogen: Synthetic peptide from C-terminus of human CD20 protein. Control Tissue: B Cell Lymphoma, Tonsil. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 931. Pathway: B Cell Markers

products description :

CD20 is a 35kDa leukocyte surface antigen consisting of four transmembrane regions and cytoplasmic N- and C-termini. CD20 is expressed primarily on B cells but has also been detected on both normal and neoplastic T cells. Activation of CD20 significantly increases DNA synthesis and is thought to involve basic helix-loop-helix leucine zipper transcription factors.

ncbi gi num :

23110987

ncbi acc num :

NP_068769.2

ncbi gb acc num :

NM_021950.3

ncbi mol weight :

35 kDa

ncbi pathways :

Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489

ncbi summary :

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]

size :

1 ml

price :

750 USD