Rabbit Anti-Human c-erbB3 Monoclonal Antibody (Clone SP71) | MyBioSource MBS302558 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human c-erbB3 Monoclonal Antibody (Clone SP71)

catalog :

MBS302558

quantity :

0.5 ml

price :

485 USD

clonality :

monoclonal

host :

rabbit

reactivity :

human, rat

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS302558

products type :

Antibody

products full name :

Rabbit Anti-Human c-erbB3 Monoclonal Antibody (Clone SP71)

products short name :

c-erbB-3/HER-3

other names :

receptor tyrosine-protein kinase erbB-3 isoform s; Receptor tyrosine-protein kinase erbB-3; receptor tyrosine-protein kinase erbB-3; human epidermal growth factor receptor 3; proto-oncogene-like protein c-ErbB-3; tyrosine kinase-type cell surface receptor HER3; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3; erb-b2 receptor tyrosine kinase 3; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3

products gene name :

c-erbB-3/HER-3

other gene names :

ERBB3; ERBB3; HER3; LCCS2; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; HER3

uniprot entry name :

ERBB3_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

SP71

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Mouse, Rat

sequence length :

183

form :

0.5 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Small Intestine

other info2 :

Immunogen: Synthetic peptide corresponding to internal region of human c-erbB-3. Control Tissue: Small Intestine. Cellular Localization: Membrane. Entrez Gene Code: 2065. Pathway: EGFR Family

products description :

The gene for c-erbB3/HER-3 is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6.2kb mRNA which is translated into a 160/180kDa glycoprotein. The c-erbB3/HER-3 gene encodes secreted as well as transmembrane receptor tyrosine kinase due to alternative splicing. The c-erbB3 is overexpressed in a variety of tumors including breast, stomach, pancreas, and colon.

ncbi gi num :

54792102

ncbi acc num :

NP_001005915.1

ncbi gb acc num :

NM_001005915.1

ncbi mol weight :

185 kDa

ncbi pathways :

Adaptive Immune System Pathway 366160!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Constitutive PI3K/AKT Signaling In Cancer Pathway 685535!!DAP12 Interactions Pathway 685549!!DAP12 Signaling Pathway 685550!!Disease Pathway 530764!!Downregulation Of ERBB2:ERBB3 Signaling Pathway 530740!!Downstream Signal Transduction Pathway 106385!!Downstream Signaling Events Of B Cell Receptor (BCR) Pathway 576250

ncbi summary :

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

size :

0.5 ml

price :

485 USD