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antibody

Rabbit Anti-Human S6 Ribosomal Protein (phospho S235/236) Monoclonal Antibody (Clone SP50)

MBS302525

0.1 ml

265 USD

monoclonal

rabbit

chicken, human, dog, rat, mouse

immunohistochemistry - paraffin section, immunohistochemistry

MBS302525

Antibody

Rabbit Anti-Human S6 Ribosomal Protein (phospho S235/236) Monoclonal Antibody (Clone SP50)

S6 Ribosomal

40S ribosomal protein S6; 40S ribosomal protein S6; 40S ribosomal protein S6; phosphoprotein NP33; ribosomal protein S6; Phosphoprotein NP33

RPS6; RPS6; S6; N/A

RS6_HUMAN

Monoclonal

IgG

SP50

Rabbit

Human. Predicted Reactivity: Bovine, Chicken, Dog, Frog, Mouse, Pig, Rabbit, Rat

249

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

Immunohistochemistry (IHC) Paraffin

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Breast Ductal Carcinoma

Immunogen: Synthetic peptide derived from the region of human S6 ribosomal that contains phosphorylated serines 235 and 236. Control Tissue: Breast Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 6194. Pathway: Translational Control

40S ribosomal protein S6 (also known as RPS6) is a ~31 kDa substrate of p70 S6 kinase (p70S6K) and a major component of translational machinery involved in protein synthesis, cell growth, proliferation, and metabolism. RPS6 undergoes phosphorylation on multiple serines in the carboxyl terminal region in the order 236-->235-->240-->244-->247, due to the positions of these amino acid residues on the alpha-helix. Hyperphosphorylation of RPS6 stimulates protein synthesis that mediates progression through the cell cycle.

17158044

NP_001001.2

NM_001010.2

31 kDa

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway 105970!!B Cell Receptor Signaling Pathway 198909!!BDNF Signaling Pathway 712093!!Cap-dependent Translation Initiation Pathway 105967!!Cytoplasmic Ribosomal Proteins Pathway 198853!!Disease Pathway 530764!!ErbB1 Downstream Signaling Pathway 138057!!Eukaryotic Translation Elongation Pathway 105976!!Eukaryotic Translation Initiation Pathway 105966!!Eukaryotic Translation Termination Pathway 105978

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

0.1 ml

265 USD