Rabbit Anti-Human 14-3-3 beta Polyclonal Antibody | MyBioSource MBS302420 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human 14-3-3 beta Polyclonal Antibody

catalog :

MBS302420

quantity :

0.5 ml

price :

315 USD

clonality :

polyclonal

host :

rabbit

reactivity :

chicken, human, rat, mouse

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS302420

products type :

Antibody

products full name :

Rabbit Anti-Human 14-3-3 beta Polyclonal Antibody

products short name :

14-3-3 beta

other names :

14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 alpha; brain protein 14-3-3, beta isoform; epididymis secretory protein Li 1; protein 1054; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1

products gene name :

14-3-3 beta

other gene names :

YWHAB; YWHAB; HS1; GW128; YWHAA; KCIP-1; HEL-S-1; KCIP-1

uniprot entry name :

1433B_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Chicken, Frog, Mouse, Rat

sequence length :

246

form :

0.5 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Brain. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Jurkat Cell Lysate

other info2 :

Immunogen: Synthetic peptide from N-terminus of human 14-3-3 beta. Control Tissue: Brain, Prostate Carcinoma, Liver Carcinoma. Cellular Localization: Cytoplasm. Entrez Gene Code: 7529. Pathway: Signal Transduction

products description :

At least 7 mammalian isoforms comprise the 14-3-3 family of signaling intermediates. 14-3-3 molecules are highly conserved, expressed in a broad range of tissues and cell types and were first discovered as a series of highly abundant proteins in brain tissue. There is increasing evidence that 14-3-3 proteins are critical to cell transformation and mitotic signaling.

ncbi gi num :

4507949

ncbi acc num :

NP_003395.1

ncbi gb acc num :

NM_003404.4

ncbi mol weight :

30 kDa

ncbi pathways :

ARMS-mediated Activation Pathway 106466!!Activation Of BAD And Translocation To Mitochondria Pathway 105659!!Activation Of BH3-only Proteins Pathway 105658!!Adaptive Immune System Pathway 366160!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Apoptosis Pathway 105648!!Axon Guidance Pathway 105688!!Butyrate Response Factor 1 (BRF1) Destabilizes MRNA Pathway 187213!!Calcium Regulation In The Cardiac Cell Pathway 198906!!Cell Cycle Pathway 198811

ncbi summary :

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

size :

0.5 ml

price :

315 USD

more info or order :

MyBioSource product webpage