Rabbit Anti-Human Occludin Polyclonal Antibody | MyBioSource MBS302417 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Occludin Polyclonal Antibody

catalog :

MBS302417

quantity :

0.5 ml

price :

315 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, rat, dog, mouse

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS302417

products type :

Antibody

products full name :

Rabbit Anti-Human Occludin Polyclonal Antibody

products short name :

Occludin

other names :

occludin isoform a; Occludin; occludin; phosphatase 1, regulatory subunit 115; tight junction protein occludin; occludin; N/A

other gene names :

OCLN; OCLN; BLCPMG; PPP1R115; N/A

uniprot entry name :

OCLN_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog, Hamster, Mouse, Pig, Rabbit, Rat

sequence length :

522

form :

0.5 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:50. IHC Positive Control: Lung. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Kidney Cell Lysate

other info2 :

Immunogen: Synthetic peptide corresponding to C-terminus of human occludin. Control Tissue: Lung. Cellular Localization: Membrane. Entrez Gene Code: 4950. Pathway: Cell Junctions

products description :

Occludin is a 65kDa protein that can exist in a variety of phosphorylated forms, ranging up to approximately 82kDa. This phosphorylation is thought to be involved in regulating both the localization and the function of occludin. Polyunsaturated fatty acids are known to up-regulate occludin expression, increasing the transendothelial cell resistance and reducing the cellular permeability to large molecules. The level of occludin varies greatly depending on tissue; in brain tissue, occludin is highly and continuously expressed at cell-cell contact sites, whereas non-neural tissues show lower expression and discontinuous distribution.

ncbi gi num :

327478414

ncbi acc num :

NP_001192183.1

ncbi gb acc num :

NM_001205254.1

ncbi mol weight :

65 kDa

ncbi pathways :

Apoptosis Pathway 105648!!Apoptotic Cleavage Of Cell Adhesion Proteins Pathway 105680!!Apoptotic Cleavage Of Cellular Proteins Pathway 105678!!Apoptotic Execution Phase Pathway 105677!!Cell Adhesion Molecules (CAMs) Pathway 83069!!Cell Adhesion Molecules (CAMs) Pathway 480!!Hepatitis C Pathway 173973!!Hepatitis C Pathway 173907!!Leukocyte Transendothelial Migration Pathway 83083!!Leukocyte Transendothelial Migration Pathway 494

ncbi summary :

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

size :

0.5 ml

price :

315 USD

more info or order :

MyBioSource product webpage