Rabbit Anti-Human SMAD3 Polyclonal Antibody | MyBioSource MBS302383 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human SMAD3 Polyclonal Antibody

catalog :

MBS302383

quantity :

0.5 ml

price :

315 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat, dog

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS302383

products type :

Antibody

products full name :

Rabbit Anti-Human SMAD3 Polyclonal Antibody

products short name :

SMAD3

other names :

mothers against decapentaplegic homolog 3 isoform 2; Mothers against decapentaplegic homolog 3; mothers against decapentaplegic homolog 3; MAD homolog 3; MAD, mothers against decapentaplegic homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; hMAD-3; hSMAD3; mad homolog JV15-2; mad protein homolog; mad3; SMAD family member 3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

products gene name :

SMAD3

other gene names :

SMAD3; SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; MADH3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD 3; Smad3; hSMAD3

uniprot entry name :

SMAD3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Chicken, Dog, Mouse, Pig, Rat

sequence length :

320

form :

0.5 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Normal Breast, Breast Carcinoma

other info2 :

Immunogen: Synthetic peptide corresponding to internal region of human SMAD3. Control Tissue: Breast, Breast Carcinoma. Cellular Localization: Nucleus. Entrez Gene Code: 4088. Pathway: Transcriptional Regulators

products description :

SMAD proteins mediate signaling events leading to transcriptional activation initiated by members of the TGF-beta superfamily. Biological activities mediated by SMAD proteins include cell growth and morphogenesis, development, and immune response. A reduction in SMAD2/3 signaling enhances tumorigenesis, but also suppresses metastasis of breast cancer cell lines.

ncbi gi num :

223029440

ncbi acc num :

NP_001138574.1

ncbi gb acc num :

NM_001145102.1

ncbi mol weight :

46 kDa

ncbi pathways :

AGE/RAGE Pathway 698754!!Activin Signaling Pathway 1084757!!Activin Signaling Pathway 1108220!!Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Adipogenesis Pathway 198832!!Alpha6-Beta4 Integrin Signaling Pathway 198807!!Androgen Receptor Signaling Pathway 198806!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054

ncbi summary :

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

size :

0.5 ml

price :

315 USD