Rabbit Anti-Human Arginase-1 Polyclonal Antibody | MyBioSource MBS302353 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human Arginase-1 Polyclonal Antibody

catalog :

MBS302353

quantity :

0.1 mL (Concentrate)

price :

180 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS302353

products type :

Antibody

products full name :

Rabbit Anti-Human Arginase-1 Polyclonal Antibody

products short name :

Arginase-1

other names :

arginase-1 isoform 2; Arginase-1; arginase-1; arginase, liver; liver-type arginase; type I arginase; arginase 1; Liver-type arginase; Type I arginase

other gene names :

ARG1; ARG1

uniprot entry name :

ARGI1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human

sequence length :

322

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Hepatocellular Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Molt-4 Cell Lysate

other info2 :

Immunogen: Synthetic peptide derived from the N-terminus of human arginase-1 protein. Control Tissue: Hepatocellular Carcinoma. Cellular Localization: Cytoplasm, Nucleus. Entrez Gene Code: 383. Pathway: Metabolism

products description :

Arginase-1 (ARG1) catalyzes the hydrolysis of L-arginine into ornithine and urea, a critical step for the urea cycle. Defects in ARG1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia. There are several isoforms of mammalian arginase. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Hepatocellular carcinoma usually shows higher protein expression of ARG1 than normal liver cells.

ncbi gi num :

10947139

ncbi acc num :

NP_000036.2

ncbi gb acc num :

NM_000045.3

ncbi mol weight :

35 kDa

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); IL4-mediated Signaling Events Pathway (137933); Metabolic Pathways (132956); Metabolism Pathway (477135)

ncbi summary :

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

uniprot summary :

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Amino Acid Metabolism - arginine and proline; Hydrolase; EC 3.5.3.1. Chromosomal Location of Human Ortholog: 6q23. Cellular Component: extracellular space; neuron projection; cell soma; cytoplasm; cytosol; nucleus. Molecular Function: manganese ion binding; arginase activity. Biological Process: response to drug; mammary gland involution; maternal process involved in pregnancy; response to herbicide; response to amino acid stimulus; liver development; response to vitamin A; response to manganese ion; response to selenium ion; response to vitamin E; response to cadmium ion; response to zinc ion; response to methylmercury; arginine catabolic process; positive regulation of endothelial cell proliferation; response to axon injury; collagen biosynthetic process; urea cycle; response to amine stimulus; lung development. Disease: Argininemia

size1 :

0.1 mL (Concentrate)

price1 :

180 USD

size2 :

7 mL (RTU)

price2 :

290

size3 :

0.5 mL (Concentrate)

price3 :

330

size4 :

1 mL (Concentrate)

price4 :

495