Rabbit Anti-Human CD68 Polyclonal Antibody | MyBioSource MBS302059 product information

product summary

request information :

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human CD68 Polyclonal Antibody

catalog :

MBS302059

quantity :

0.1 ml

price :

170 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS302059

products type :

Antibody

products full name :

Rabbit Anti-Human CD68 Polyclonal Antibody

products short name :

CD68

other names :

macrosialin isoform B; Macrosialin; macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1; CD68 molecule; Gp110; CD_antigen: CD68

products gene name :

CD68

other gene names :

CD68; CD68; GP110; LAMP4; SCARD1; N/A

uniprot entry name :

CD68_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Rabbit

sequence length :

327

form :

0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Tonsil, Lung. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:50. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: SH-SY5Y Cell Lysate, Raji Cell Lysate

other info2 :

Immunogen: Synthetic peptide near the C-terminus of human CD68 protein. Control Tissue: Tonsil, Lung. Cellular Localization: Cytoplasm. Entrez Gene Code: 968. Pathway: Macrophage

products description :

CD68 is an intracellular glycoprotein mainly expressed on macrophages and monocytes. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is also expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma.

ncbi gi num :

91199550

ncbi acc num :

NP_001035148.1

ncbi gb acc num :

NM_001040059.1

ncbi mol weight :

37 kDa Protein (calculated); 110 kDa (apparent)

ncbi pathways :

Lysosome Pathway 99052!!Lysosome Pathway 96865

ncbi summary :

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

size :

0.1 ml

price :

170 USD

more info or order :

MyBioSource product webpage