catalog number :
MBS301986
products full name :
Rabbit Anti-Human Uroplakin III Polyclonal Antibody
products short name :
Uroplakin III
other names :
uroplakin-3a isoform 2; Uroplakin-3a; uroplakin-3a; uroplakin 3; uroplakin III; uroplakin 3A; Uroplakin III; UPIII
other gene names :
UPK3A; UPK3A; UP3A; UPK3; UPIII; UPIIIA; UPK3; UP3a; UPIII
uniprot entry name :
UPK3A_HUMAN
reactivity :
Human. Predicted Reactivity: Bovine, Dog, Mouse, Pig, Rabbit, Rat
form :
0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
storage stability :
Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
tested application :
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
app notes :
Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilutions: If using the concentrate format of this product, dilute the antibody 1:100 prior to use. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Primary Antibody: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: 1:100. IHC Positive Control: Bladder, Bladder Carcinoma. Western Blot . Recommended Starting Protocol: Dilute the antibody 1:100. Incubate for 1 hour at room temperature. The dilution is an estimate; actual results may differ because of variability in methods and protocols. Optimal dilution and procedure should be determined by the end user. Western Blot Positive Control: Bladder Cell Lysate
other info2 :
Immunogen: Synthetic peptide corresponding to C-terminus of human uroplakin III. Control Tissue: Bladder, Bladder Carcinoma. Cellular Localization: Cytoplasm, Membrane. Entrez Gene Code: 7380. Pathway: Cancer Markers
products description :
Uroplakins are specific differentiation products of terminally differentiated superficial urothelial cells. Uroplakin III is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas.
ncbi acc num :
NP_001161046.1
ncbi gb acc num :
NM_001167574.1
ncbi summary :
This gene encodes a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Mutations in this gene may be associated with renal adysplasia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]