Rabbit Anti-Human PIM-1 Polyclonal Antibody | MyBioSource MBS301937 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Rabbit Anti-Human PIM-1 Polyclonal Antibody

catalog :

MBS301937

quantity :

7 ml

price :

285 USD

clonality :

polyclonal

host :

rabbit

reactivity :

human, mouse, rat, dog

application :

immunohistochemistry, immunohistochemistry - paraffin section

product information

catalog number :

MBS301937

products type :

Antibody

products full name :

Rabbit Anti-Human PIM-1 Polyclonal Antibody

products short name :

PIM-1

other names :

serine/threonine-protein kinase pim-1 isoform 1; Serine/threonine-protein kinase pim-1; serine/threonine-protein kinase pim-1; Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1; Pim-1 proto-oncogene, serine/threonine kinase

products gene name :

PIM-1

other gene names :

PIM1; PIM1; PIM

uniprot entry name :

PIM1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human. Predicted Reactivity: Bovine, Dog, Mouse, Rabbit, Rat

sequence length :

404

form :

7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

storage stability :

Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.

tested application :

Immunohistochemistry (IHC) Paraffin

app notes :

Immunohistochemistry Procedure . Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM EDTA, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. Immunohistochemistry Dilution: Ready To Use. IHC Positive Control: Prostate Adenoarcinoma

other info2 :

Immunogen: Synthetic peptide derived from internal region of human PIM-1 protein. Control Tissue: Bone Marrow. Cellular Localization: Cytoplasm, Membrane, Nucleus. Entrez Gene Code: 5292. Pathway: Signal Transduction

products description :

PIM-1 is a member of the serine/threonine kinase PIM oncogene family. PIM-1 has been implicated in lymphomagenesis, cell proliferation, apoptosis, differentiation and tumourigenesis. The PIM-1 protein kinase is upregulated in prostate cancer.

ncbi gi num :

342307046

ncbi acc num :

NP_001230115.1

ncbi gb acc num :

NM_001243186.1

ncbi mol weight :

45 kDa(Isoform 1) and 36 kDa (Isoform 2)

ncbi pathways :

Acute Myeloid Leukemia Pathway 83117!!Acute Myeloid Leukemia Pathway 529!!C-MYB Transcription Factor Network Pathway 138073!!IL-5 Signaling Pathway 198770!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488!!MicroRNAs In Cancer Pathway 852705!!MicroRNAs In Cancer Pathway 852928!!Role Of Calcineurin-dependent NFAT Signaling In Lymphocytes Pathway 137953!!Validated Targets Of C-MYC Transcriptional Activation Pathway 169351

ncbi summary :

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011]

size :

7 ml

price :

285 USD